Raiger Iustman Laura J, Tribelli Paula M, Ibarra José G, Catone Mariela V, Solar Venero Esmeralda C, López Nancy I
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina.
Extremophiles. 2015 Jan;19(1):207-20. doi: 10.1007/s00792-014-0700-7. Epub 2014 Oct 15.
The genome of the Antarctic bacterium Pseudomonas extremaustralis was analyzed searching for genes involved in environmental adaptability focusing on anaerobic metabolism, osmoregulation, cold adaptation, exopolysaccharide production and degradation of complex compounds. Experimental evidences demonstrated the functionality of several of these pathways, including arginine and pyruvate fermentation, alginate production and growth under cold conditions. Phylogenetic analysis along with genomic island prediction allowed the detection of genes with probable foreign origin such as those coding for acetate kinase, osmotic resistance and colanic acid biosynthesis. These findings suggest that in P. extremaustralis the horizontal transfer events and/or gene redundancy could play a key role in the survival under unfavorable conditions. Comparative genome analysis of these traits in other representative Pseudomonas species highlighted several similarities and differences with this extremophile bacterium.
对南极细菌极端南极假单胞菌（Pseudomonas extremaustralis）的基因组进行了分析，寻找参与环境适应性的基因，重点关注厌氧代谢、渗透调节、冷适应、胞外多糖产生以及复杂化合物的降解。实验证据证明了其中几条途径的功能，包括精氨酸和丙酮酸发酵、藻酸盐产生以及在低温条件下的生长。系统发育分析以及基因组岛预测使得能够检测到可能具有外源起源的基因，例如编码乙酸激酶、渗透压抗性和柯氏酸生物合成的基因。这些发现表明，在极端南极假单胞菌中，水平转移事件和/或基因冗余可能在不利条件下的生存中起关键作用。对其他代表性假单胞菌物种这些性状的比较基因组分析突出了与这种嗜极细菌的若干异同。
