Carlsen S A, Barry M, Newton K
Department of Microbiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Clin Exp Metastasis. 1990 Mar-Apr;8(2):141-51. doi: 10.1007/BF00117787.
The process of metastasis is a complex process involving numerous steps, and it is thought that cells able to complete all of these steps and form metastatic foci form a unique subpopulation of cells within the tumor. To study this metastatic subpopulation, a marker for the metastatic cells is required. We have previously described the enrichment of soybean agglutinin binding cells in tumor populations enriched for lymphatic metastasis [1]. In this study we provide evidence that the cell-surface structure binding soybean agglutinin is a neutral glycosphingolipid. Using monoclonal antibodies generated against this glycolipid, highly metastatic tumor populations were depleted of cells containing this glycolipid. These depleted cell populations were found to be equally tumorigenic to that of the untreated population but were much less metastatic. These results suggest that this glycolipid may be a useful marker for metastatic cells.
转移过程是一个涉及众多步骤的复杂过程，据认为能够完成所有这些步骤并形成转移灶的细胞在肿瘤内形成了一个独特的细胞亚群。为了研究这个转移亚群，需要一种转移细胞的标志物。我们之前已经描述了在富含淋巴转移的肿瘤群体中大豆凝集素结合细胞的富集情况[1]。在本研究中，我们提供证据表明与大豆凝集素结合的细胞表面结构是一种中性糖鞘脂。使用针对这种糖脂产生的单克隆抗体，高度转移的肿瘤群体中含有这种糖脂的细胞被耗尽。发现这些耗尽的细胞群体与未处理群体的致瘤性相同，但转移性要低得多。这些结果表明这种糖脂可能是转移细胞的一种有用标志物。
