Capuana Maurizio
IGV-Istituto di Genetica Vegetale, National Research Council (CNR), Sesto Fiorentino, Florence, Italy.
Methods Mol Biol. 2013;11013:213-21. doi: 10.1007/978-1-62703-074-8_16.
Induction of somatic embryogenesis is described in common ash (Fraxinus excelsior L.). Embryogenic tissues are obtained from immature zygotic embryos and cultured on a modified Murashige and Skoog (MS) medium containing 8.8 μM 2,4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine. Embryogenic tissue is subcultured and multiplied on medium supplemented with reduced concentration of plant growth hormones. Somatic embryos develop and mature by transfer to hormone-free medium and subsequent culture on medium containing low amount of benzyladenine. Somatic embryo germination and conversion are enhanced by cold storage at 4°C and successive transfer onto Woody Plant Medium (WPM). Fully developed plantlets are then transferred to pots and acclimatized in the greenhouse equipped with a mist system.
文中描述了欧洲白蜡树（Fraxinus excelsior L.）体细胞胚胎发生的诱导过程。胚性组织取自未成熟合子胚，并在含有8.8 μM 2,4-二氯苯氧乙酸和4.4 μM苄基腺嘌呤的改良Murashige和Skoog（MS）培养基上培养。胚性组织在添加了浓度降低的植物生长激素的培养基上进行继代培养和增殖。通过转移到无激素培养基上并随后在含有少量苄基腺嘌呤的培养基上培养，体细胞胚得以发育和成熟。在4°C下冷藏并连续转移到木本植物培养基（WPM）上可增强体细胞胚的萌发和转化。然后将完全发育的小植株转移到花盆中，并在配备喷雾系统的温室中进行驯化。
