Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karls University of Tübingen, Tübingen, Germany.
Microsurgery. 2013 Mar;33(3):216-22. doi: 10.1002/micr.22059. Epub 2012 Nov 24.
In this study, we evaluated the role of the Netrin-1 receptor UNC5b (Uncoordinated), a neuronal guidance molecule, during peripheral nerve regeneration using the mouse median nerve model.
Using Western blot analysis, we examined the expression changes of UNC5b after transection and microsurgical repair of the mouse median nerve distal to the transection site. We evaluated the histomorphometrical changes and functional recovery of the grasping force after median nerve transection and repair in wild-type (WT) mice and UNC5b(+/-) heterozygous mice.
In Western blot analysis, we could show a high increase of UNC5b in the nerve segment distal to the injury site at day 14. Histomorphometrical analysis did not show any significant differences between WT animals and heterozygous animals. Using the functional grasping test, we could demonstrate that peripheral nerve regeneration is significantly diminished in heterozygous UNC5b(+/-) mice.
By using the mouse median nerve model in transgenic animals, we demonstrate that the Netrin-1 receptor UNC5b plays an important role during peripheral nerve regeneration.
在这项研究中，我们使用小鼠正中神经模型评估了神经导向分子 UNC5b（未协调）在周围神经再生中的作用。
通过 Western blot 分析，我们检测了 UNC5b 在小鼠正中神经切断和远侧吻合后的表达变化。我们评估了 WT 小鼠和 UNC5b(+/-)杂合子小鼠正中神经切断和修复后的组织形态学变化和抓握力的功能恢复。
在 Western blot 分析中，我们可以在损伤部位 14 天的神经节段远端显示 UNC5b 的高表达。组织形态学分析显示 WT 动物和杂合子动物之间没有明显差异。使用功能抓握试验，我们可以证明 UNC5b(+/-)杂合子小鼠的周围神经再生明显减少。
通过使用转基因动物的小鼠正中神经模型，我们证明 Netrin-1 受体 UNC5b 在周围神经再生中发挥重要作用。
