Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410078, China.
Inflammation. 2013 Jun;36(3):521-8. doi: 10.1007/s10753-012-9573-z.
B cell activation mediated by cluster of differentiation (CD) molecules plays an important role in B cell-related autoimmune diseases. CD22 and CD72 have been demonstrated to act as B cell inhibitory receptors in many autoimmune diseases. Activated B cells are involved in the pathogenesis of myasthenia gravis (MG) by secretion of anti-acetylcholine receptor (AchR) antibodies. However, the roles of CD22 and CD72 on B cells of MG are unknown. In this study, we detected the expression of CD22 and CD72 on B cells of MG, compared to multiple sclerosis (MS) patient controls and healthy controls by flow cytometry and quantitative real-time polymerase transcription chain reaction. Our data demonstrated that aberrant expression of CD72 exists on B cells of MG and MS patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in MG, which suggests that CD72 may be involved in the pathogenesis of MG and MS. There were no significant differences between study patients (MG, ocular MG, generalized MG, and MS) and healthy controls.
B 细胞激活介导的分化（CD）分子在 B 细胞相关自身免疫性疾病中发挥重要作用。CD22 和 CD72 已被证明在许多自身免疫性疾病中作为 B 细胞抑制受体发挥作用。激活的 B 细胞通过分泌抗乙酰胆碱受体（AchR）抗体参与重症肌无力（MG）的发病机制。然而，CD22 和 CD72 在 MG 中的 B 细胞的作用尚不清楚。在这项研究中，我们通过流式细胞术和实时定量聚合酶转录链反应检测了 MG、多发性硬化症（MS）患者对照和健康对照者 B 细胞中 CD22 和 CD72 的表达。我们的数据表明，MG 和 MS 患者的 B 细胞存在 CD72 的异常表达，CD72 分子的表达水平与 MG 中的抗 AchR 抗体水平呈显著负相关，这表明 CD72 可能参与了 MG 和 MS 的发病机制。研究患者（MG、眼肌型 MG、全身型 MG 和 MS）与健康对照者之间无显著差异。
