Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
World J Microbiol Biotechnol. 2013 Apr;29(4):635-43. doi: 10.1007/s11274-012-1219-7. Epub 2012 Nov 27.
We analyzed methylotrophs in Bina natural vegetation (BNV), and revegetated overburden dump of four (ROBD4) and 12 years (ROBD12), at Bina coal mine in Sonbhadra district. The cultured strains identified as Pseudomonas, Acinetobacter, Stenotrophomonas and Cellvibrio (γ-Proteobacteria), Methylophilus, Ralstonia, Burkholderia (α-Proteobacteria) Methylobacterium and Inquilinus (β-Proteobacteria), Bacillus (Firmicutes) and Flexibacter (Sphingobacteria) in their 16s rRNA gene sequence similarity. The strains differed in citrate, lactose, formate, urea and xylose utilization. Methanol utilization by Stenotrophomonas, Inquilinus, Cellvibrio and Flexibacter is for first time. The preferred N- sources were proline, glutamate and nitrate for most of the strains. All strains tolerated (2.5 % NaCl) and SDS (0.2 %); 16 strains survived in crystal violet (0.01 %) and nine strains in sodium azide (0.02 %. Methylotrophic population trend was BNV > ROBD12 > ROBD4. The presence of majority of strain of BNV at ROBD12 and ROBD4 indicated restoration of soil methylotrophic functional diversity in revegetated dumps.
我们分析了比纳自然植被（BNV）和复垦过的四个（ROBD4）和 12 年（ROBD12）过煤层的甲基营养菌，地点在索尼巴德拉区的比纳煤矿。根据 16s rRNA 基因序列相似性，培养的菌株被鉴定为假单胞菌、不动杆菌、寡养单胞菌和纤维弧菌（γ-变形菌）、甲基单胞菌、罗尔斯通氏菌、伯克霍尔德菌（α-变形菌）、甲基杆菌和侵入菌（β-变形菌）、芽孢杆菌（Firmicutes）和鞘氨醇单胞菌（Sphingobacteria）。这些菌株在利用柠檬酸盐、乳糖、甲酸盐、尿素和木糖方面存在差异。第一次发现寡养单胞菌、侵入菌、纤维弧菌和 Flexibacter 能够利用甲醇。大多数菌株优先利用脯氨酸、谷氨酸和硝酸盐作为氮源。所有菌株都能耐受（2.5%NaCl）和 SDS（0.2%）；16 株菌能在结晶紫（0.01%）中存活，9 株菌能在叠氮化钠（0.02%）中存活。甲基营养菌的种群趋势是 BNV > ROBD12 > ROBD4。在 ROBD12 和 ROBD4 中发现了大多数 BNV 菌株的存在，这表明复垦堆中土壤甲基营养功能多样性得到了恢复。
