Department of Chemistry and Pharmacy, Food Chemistry, Emil Fischer Center, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
Steroids. 2013 Feb;78(2):156-60. doi: 10.1016/j.steroids.2012.11.005. Epub 2012 Nov 24.
A method was developed for the quantification of 5-α-androst-16-en-3-one in human breast milk based on application of a stable isotope dilution assay using 5α-androst-16-en-3-one-6, 6-d(2). The procedure includes extraction of the human milk by hexane with subsequent clean-up of the obtained extract by gel permeation and silica gel column chromatography. The extracted samples were analyzed by gas chromatography-mass spectrometry. Using this method 5-α-androst-16-en-3-one could be identified and for the first time quantified in a concentration range of 26-155 ng/kg in human milk.
建立了一种基于稳定同位素稀释分析的方法，应用 5α-雄烷-16-烯-3-酮-6,6-d2，对人乳中 5α-雄烷-16-烯-3-酮进行定量分析。该方法包括用正己烷提取人乳，然后用凝胶渗透和硅胶柱色谱法对提取的样品进行净化。采用气相色谱-质谱法对提取的样品进行分析。应用该方法首次在人乳中鉴定并定量检测到 5α-雄烷-16-烯-3-酮，浓度范围为 26-155ng/kg。
