Nixon A, Mallet A I, Gower D B
Division of Biochemistry, United Medical School, Guy's Hospital, London, England.
J Steroid Biochem. 1988 May;29(5):505-10. doi: 10.1016/0022-4731(88)90185-9.
Five 16-androstenes have been simultaneously quantified in extracts of the axillary hair of men (age range 18-40 years) using combined capillary gas chromatography-mass spectrometry, with specific ion monitoring. Quantities found (pmol/mg.hair, with approximate 24-h totals in parentheses) were: 5 alpha-androst-16-en-3-one, 0-15 (0-433); 4, 16-androstadien-3-one, 0-143 (0-4103); 5,16-androstadien-3 beta-ol, 0-3.5 (0-728); 5 alpha-androst-16-en-3 alpha-ol, 0-17 (0-1752) and 5 alpha-androst-16-en-3 beta-ol, 0-4 (0-416). There were no significant relationships with age of the subjects for any of the steroids measured but significant relationships were found between the amounts of the two ketones and between 5 alpha-androst-16-en-3 alpha- and 3 beta-ols. These findings may indicate the existence of a pathway of metabolism in axillary bacteria in which 4,16-androstadien-3-one is reduced to 5 alpha-androst-16-en-3-one and thence to the 3 alpha- and 3 beta-alcohols. The data are discussed in the context of axillary odour because of the low olfactory thresholds of several of the 16-androstenes measured and because of the relatively large quantities found in some subjects.
采用毛细管气相色谱 - 质谱联用及特定离子监测技术，同时对18至40岁男性腋毛提取物中的5种16 - 雄烯进行了定量分析。所测含量（pmol/mg毛发，括号内为约24小时总量）分别为：5α - 雄甾 - 16 - 烯 - 3 - 酮，0 - 15（0 - 433）；4,16 - 雄二烯 - 3 - 酮，0 - 143（0 - 4103）；5,16 - 雄二烯 - 3β - 醇，0 - 3.5（0 - 728）；5α - 雄甾 - 16 - 烯 - 3α - 醇，0 - 17（0 - 1752）和5α - 雄甾 - 16 - 烯 - 3β - 醇，0 - 4（0 - 416）。所测任何一种甾体与受试者年龄均无显著相关性，但发现两种酮类物质的含量之间以及5α - 雄甾 - 16 - 烯 - 3α - 醇和3β - 醇之间存在显著相关性。这些发现可能表明腋部细菌中存在一条代谢途径，其中4,16 - 雄二烯 - 3 - 酮先还原为5α - 雄甾 - 16 - 烯 - 3 - 酮，进而还原为3α - 醇和3β - 醇。鉴于所测几种16 - 雄烯的嗅觉阈值较低且部分受试者中含量相对较高，故结合腋部气味对这些数据进行了讨论。
