早期分化的被子植物 Trithuria（Hydatellaceae）生殖结构中阿拉伯半乳聚糖蛋白（AGPs）的免疫定位。
Immunolocalization of arabinogalactan proteins (AGPs) in reproductive structures of an early-divergent angiosperm, Trithuria (Hydatellaceae).
机构信息
Departamento de Biologia, Universidade do Porto, Porto, Portugal.
出版信息
Ann Bot. 2013 Feb;111(2):183-90. doi: 10.1093/aob/mcs256. Epub 2012 Nov 27.
BACKGROUND AND AIMS
Trithuria is the sole genus of Hydatellaceae, a family of the early-divergent angiosperm lineage Nymphaeales (water-lilies). In this study different arabinogalactan protein (AGP) epitopes in T. submersa were evaluated in order to understand the diversity of these proteins and their functions in flowering plants.
METHODS
Immunolabelling of different AGPs and pectin epitopes in reproductive structures of T. submersa at the stage of early seed development was achieved by immunofluorescence of specific antibodies.
KEY RESULTS
AGPs in Trithuria pistil tissues could be important as structural proteins and also as possible signalling molecules. Intense labelling was obtained with anti-AGP antibodies both in the anthers and in the intine wall, the latter associated with pollen tube emergence.
CONCLUSIONS
AGPs could play a significant role in Trithuria reproduction, due to their specific presence in the pollen tube pathway. The results agree with labellings obtained for Arabidopsis and confirms the importance of AGPs in angiosperm reproductive structures as essential structural components and probably important signalling molecules.
背景与目的
水玉簪科（Hydatellaceae）是单子叶植物睡莲目（Nymphaeales，即“水百合目”）中一个早期分化的科，其中仅包含水玉簪属（Trithuria）这一属。本研究旨在评估水玉簪属（Trithuria）中不同的阿拉伯半乳糖蛋白（AGP）表位，以了解这些蛋白在开花植物中的多样性及其功能。
方法
通过特异性抗体的免疫荧光法，对水玉簪属（Trithuria）早期种子发育阶段生殖结构中的不同 AGP 和果胶表位进行免疫标记。
主要结果
雌蕊组织中的 AGP 可能作为结构蛋白，也可能作为信号分子发挥重要作用。在花药和内绒毡层中均获得了强烈的 AGP 抗体标记，后者与花粉管的萌发有关。
结论
AGP 可能在水玉簪属（Trithuria）的繁殖中发挥重要作用，因为它们在花粉管途径中具有特异性。这些结果与拟南芥的标记结果一致，证实了 AGP 在被子植物生殖结构中作为重要结构成分的重要性，并且可能是重要的信号分子。
Zotero 插件