Department of Subtropical Fruit Crops, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM la Mayora-UMA-CSIC), Avenida Dr. Wienberg, s/n., 29750, Algarrobo-Costa, Málaga, Spain.
Plant Reprod. 2018 Dec;31(4):385-397. doi: 10.1007/s00497-018-0342-5. Epub 2018 Jun 22.
The mango (Mangifera indica) is a woody perennial crop currently cultivated worldwide in regions with tropical and subtropical climates. Despite its importance, an essential process such as pollen development, and, specifically, cell wall composition that influences crosstalk between somatic cells and the male germline, is still poorly understood in this species and in the Anacardiaceae as a whole. A detailed understanding of this process is particularly important to know the effect of low temperatures during flowering on pollen development that can be a limiting factor for fertilization and fruit set. To fill this gap, we performed a thorough study on the cell wall composition during pollen development in mango. The results obtained reveal a clear differentiation of the cell wall composition of the male germline by pectins, AGPs and extensins from the early developmental stages during microsporogenesis and microgametogenesis reflecting a restricted communication between the male germline and the surrounding somatic cells that is very sensitive to low temperatures. The combination of the results obtained provides an integrated study on cell wall composition of the male germline in mango that reveals the crucial role of the sporophyte and the gametophyte and the vulnerability of the process to low temperatures.
芒果（Mangifera indica）是一种木本多年生作物，目前在热带和亚热带气候地区广泛种植。尽管芒果很重要，但花粉发育等基本过程，特别是影响体细胞和雄性生殖细胞之间串扰的细胞壁组成，在该物种和漆树科中仍知之甚少。详细了解这一过程对于了解开花期低温对花粉发育的影响尤为重要，因为低温可能是受精和结实的限制因素。为了填补这一空白，我们对芒果花粉发育过程中的细胞壁组成进行了深入研究。研究结果表明，在小孢子发生和小配子发生的早期发育阶段，雄性生殖细胞的细胞壁组成明显分化为果胶、AGP 和伸展蛋白，反映了雄性生殖细胞与周围体细胞之间的交流受到限制，这种交流对低温非常敏感。综合研究结果揭示了芒果雄性生殖细胞细胞壁组成的重要性，反映了孢子体和配子体的关键作用，以及该过程对低温的脆弱性。
