嗜碱性粒细胞活化试验的性能和应用更新。
Update on the performance and application of basophil activation tests.
机构信息
Division of Allergy and Clinical Immunology, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA.
出版信息
Curr Allergy Asthma Rep. 2013 Feb;13(1):101-9. doi: 10.1007/s11882-012-0324-x.
Abstract
The basophil activation test (BAT) is a flow-cytometry-based functional assay that assesses the degree of cell activation after exposure to a stimuli. Though no standardized technique currently exists, recent advances have improved the performance of this assay, including identification of new basophil-specific markers and comparisons of the expression of CD63 to CD203c during activation. The basophil activation test has also been validated for many IgE-mediated disease conditions, which have been extensively reviewed elsewhere. This review focuses on the most recent applications of this test to the diagnosis of allergy to drugs, foods, venoms, and pollens, and the evolving role of the BAT in monitoring immunotherapy.
摘要
嗜碱性粒细胞激活试验（BAT）是一种基于流式细胞术的功能检测方法，用于评估细胞在暴露于刺激物后的激活程度。虽然目前尚无标准化技术，但最近的进展已经提高了该检测方法的性能，包括鉴定新的嗜碱性粒细胞特异性标志物以及在激活过程中比较 CD63 与 CD203c 的表达。嗜碱性粒细胞激活试验也已经在许多 IgE 介导的疾病条件下得到验证，这些内容在其他地方已经有广泛的综述。本文重点介绍了该检测方法在药物、食物、毒液和花粉过敏诊断中的最新应用，以及嗜碱性粒细胞激活试验在免疫治疗监测中的作用不断演变。
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