Schneyer A L, Mason A J, Burton L E, Ziegner J R, Crowley W F
Department of Medicine, Massachusetts General Hospital, Boston 02114.
J Clin Endocrinol Metab. 1990 Apr;70(4):1208-12. doi: 10.1210/jcem-70-4-1208.
The specificity of the most widely used heterologous RIA for human serum inhibition was examined with regard to its cross-reactivity with the alpha-subunit of inhibin. Using a mixture of recombinant alpha inhibin proteins, including precursor and mature forms, the epitopic specificity of this antibody was localized entirely to the alpha-subunit. Furthermore, varying degrees of alpha inhibin immunoactivity were demonstrated in serum from normal subjects and patients with various reproductive disorders by both Western blot and RIA. These results demonstrate that data obtained using this RIA should be interpreted with caution as to the degree to which they reflect the presence of intact dimeric inhibin vs. alpha inhibin proteins in the circulation.
针对最广泛使用的人血清抑制异源放射免疫分析（RIA）与抑制素α亚基的交叉反应性，对其特异性进行了检测。使用包括前体和成熟形式在内的重组α抑制素蛋白混合物，将该抗体的表位特异性完全定位到α亚基上。此外，通过蛋白质印迹法（Western blot）和放射免疫分析，在正常受试者和患有各种生殖系统疾病患者的血清中均显示出不同程度的α抑制素免疫活性。这些结果表明，对于使用该放射免疫分析获得的数据，在解释其反映循环中完整二聚体抑制素与α抑制素蛋白存在程度时应谨慎。
