Xia Zhi-Chao, Kong Chui-Hua, Wang Peng, Chen Long-Chi, Wang Si-Long
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Ying Yong Sheng Tai Xue Bao. 2012 Aug;23(8):2135-40.
By using dilution plate, fumigation extraction, and phospholipid fatty acid (PLFA) methods, this paper studied the quantities of soil microbial populations and the characteristics of soil microbial community structure in a Chinese fir (Cunninghamia lanceolata) plantation converted from an evergreen broadleaved forest. The results showed that, during the vegetation change from evergreen broadleaved forest to Chinese fir plantation, the microbial biomass carbon and the quantities of culturable bacteria and actinomyces were decreased. The total PLFAs, bacterial PLFAs, and fungi PLFAs in the woodland soil from Chinese fir plantation were decreased by 49.4%, 52.4%, 46.6%, simultaneously. And G+ and G- bacterial PLFAs in Chinese fir plantation were lower than in evergreen broadleaved forest. As compared with those in rhizosphere soil from Chinese fir plantation, the microbial biomass carbon and the quantities of culturable bacteria and actinomyces in bulk soil were decreased. The total PLFAs, bacterial PLFAs, and G+ and G- bacterial PLFAs in the rhizosphere soil were increased, while the ratio of fungal to bacterial PLFAs was lowered. The principal component analysis of the soil microbial community structure indicated that the first principal component (PC1) and the second principal component (PC2) together accounted for 78.2% of total variation of soil microbial community structure. This study showed there were some differences in the soil microbial community structure between evergreen broadleaved forest and Chinese fir plantation.
本文采用稀释平板法、熏蒸提取法和磷脂脂肪酸（PLFA）分析法，研究了由常绿阔叶林转变而来的杉木人工林中土壤微生物种群数量及土壤微生物群落结构特征。结果表明，在从常绿阔叶林向杉木人工林转变的植被演替过程中，微生物生物量碳以及可培养细菌和放线菌数量均减少。杉木人工林林地土壤中总PLFAs、细菌PLFAs和真菌PLFAs同时分别下降了49.4%、52.4%、46.6%。而且，杉木人工林中G+和G-细菌PLFAs低于常绿阔叶林。与杉木人工林根际土壤相比，非根际土壤中微生物生物量碳以及可培养细菌和放线菌数量减少。根际土壤中总PLFAs、细菌PLFAs以及G+和G-细菌PLFAs增加，而真菌与细菌PLFAs的比值降低。土壤微生物群落结构的主成分分析表明，第一主成分（PC1）和第二主成分（PC2）共同解释了土壤微生物群落结构总变异的78.2%。本研究表明，常绿阔叶林和杉木人工林之间土壤微生物群落结构存在一些差异。
