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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
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Thioesterase superfamily member 2/acyl-CoA thioesterase 13 (Them2/Acot13) regulates hepatic lipid and glucose metabolism.硫酯酶超家族成员 2/酰基辅酶 A 硫酯酶 13（Them2/Acot13）调节肝脏脂质和葡萄糖代谢。
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Mice lacking Pctp /StarD2 exhibit increased adaptive thermogenesis and enlarged mitochondria in brown adipose tissue.缺乏 Pctp/StarD2 的小鼠表现出棕色脂肪组织中适应性生热增加和线粒体增大。
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Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs.硫酯酶超家族成员2（Them2）/酰基辅酶A硫酯酶13（Acot13）：一种对长链脂肪酰基辅酶A具有选择性的同四聚体热狗折叠硫酯酶。
Biochem J. 2009 Jun 26;421(2):311-22. doi: 10.1042/BJ20090039.
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The mechanisms of human hotdog-fold thioesterase 2 (hTHEM2) substrate recognition and catalysis illuminated by a structure and function based analysis.基于结构与功能分析揭示人类热狗折叠硫酯酶2（hTHEM2）的底物识别和催化机制
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7
Interacting proteins dictate function of the minimal START domain phosphatidylcholine transfer protein/StarD2.相互作用蛋白决定最小START结构域磷脂酰胆碱转移蛋白/StarD2的功能。
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8
Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule.人类硫酯酶超家族成员2（hTHEM2）与β-微管蛋白共定位于微管上。
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9
Crystal structure of human thioesterase superfamily member 2.人类硫酯酶超家族成员2的晶体结构
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斑马鱼THEM2的分子克隆、表达、纯化及晶体学分析

Molecular cloning, expression, purification and crystallographic analysis of zebrafish THEM2.

作者信息

Li Han, Gao Feng, Yu Shanshan, Jia Minze, Gong Weimin

机构信息

Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1525-8. doi: 10.1107/S1744309112043813. Epub 2012 Nov 14.

DOI:10.1107/S1744309112043813
PMID:23192039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3509980/
Abstract

Thioesterase superfamily member 2 (THEM2) is essential for cell proliferation of mammalian cells. It belongs to the hotdog-fold thioesterase superfamily and catalyzes the hydrolysis of the thioester bonds of acyl-CoA in vitro. In this study, THEM2 protein from zebrafish (fTHEM2) was expressed in Escherichia coli and purified by Ni-affinity and gel-filtration chromatography. fTHEM2 crystals were obtained using the sitting-drop vapour-diffusion method with PEG 10 000 as precipitant. X-ray diffraction data were collected to 1.80 Å resolution using a synchrotron-radiation source. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=77.1, b=74.4, c=96.6 Å, β=93.7°.

摘要

硫酯酶超家族成员2（THEM2）对哺乳动物细胞的增殖至关重要。它属于热狗折叠硫酯酶超家族，在体外催化酰基辅酶A硫酯键的水解。在本研究中，斑马鱼的THEM2蛋白（fTHEM2）在大肠杆菌中表达，并通过镍亲和层析和凝胶过滤层析进行纯化。使用以聚乙二醇10000作为沉淀剂的坐滴气相扩散法获得了fTHEM2晶体。利用同步辐射源收集了分辨率为1.80 Å的X射线衍射数据。晶体属于单斜空间群C2，晶胞参数为a = 77.1、b = 74.4、c = 96.6 Å，β = 93.7°。