来自拟南芥的4-香豆酸:辅酶A连接酶的结晶及初步X射线分析。
Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana.
作者信息
Morita Hiroyuki, Mori Takahiro, Wanibuchi Kiyofumi, Kato Ryohei, Sugio Shigetoshi, Abe Ikuro
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):409-11. doi: 10.1107/S1744309111002181. Epub 2011 Feb 25.
Abstract
4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a=91.6, b=55.5, c=124.4 Å, α=γ=90.0, β=111.1°.
摘要
来自拟南芥的4-香豆酸：辅酶A连接酶2（4CL2）通过形成4-香豆酸-AMP催化ATP依赖性的4-香豆酰辅酶A硫酯的形成。重组4CL2蛋白在大肠杆菌中表达，并通过坐滴气相扩散法结晶。晶体属于空间群P2(1)，晶胞参数为a = 91.6、b = 55.5、c = 124.4 Å，α = γ = 90.0，β = 111.1°。
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