来自耐辐射球菌的DdrB-DNA复合物的结晶
Crystallization of the DdrB-DNA complex from Deinococcus radiodurans.
作者信息
Sugiman-Marangos Seiji, Junop Murray
机构信息
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1534-7. doi: 10.1107/S1744309112044041. Epub 2012 Nov 14.
Abstract
The remarkable ability of members of the Deinococcus family to recover from extreme DNA damage is in part owing to their robust DNA-repair mechanisms. Of particular interest is their ability to repair hundreds of double-strand DNA breakages through a rapid and efficient mechanism involving novel proteins that are uniquely found in Deinococcus spp. One such protein, DdrB, which is thought to play a role early in DSB repair, has been crystallized in complex with ssDNA and data have been collected to 2.3 Å resolution.
摘要
嗜放射菌科成员从极端DNA损伤中恢复的卓越能力部分归功于其强大的DNA修复机制。特别值得关注的是，它们能够通过一种快速有效的机制修复数百处双链DNA断裂，该机制涉及嗜放射菌属中特有的新型蛋白质。其中一种这样的蛋白质DdrB，被认为在双链断裂修复早期发挥作用，已与单链DNA形成复合物结晶，并且已收集到分辨率为2.3 Å的数据。
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