Baker R C, Tucker M, Clay K L
Department of Pharmacology, University of Colorado Health Sciences Center, Denver.
J Pharmacol Exp Ther. 1990 Mar;252(3):1028-33.
Ethanol was examined for its effects on zymosan phagocytosis and synthesis of platelet-activating factor (PAF) using a clonal macrophage cell line. PAF, identified as 1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine, is a biologically active ether phospholipid produced by various cells types. PAF also activates a variety of inflammatory cells including the cells which produce PAF. Ethanol at 20 to 100 mM inhibited phagocytosis of unopsonized zymosan and concomitantly decreased PAF concentration in the stimulated macrophages. In contrast, in cells not stimulated with zymosan, ethanol increased the recoverable PAF. Ethanol, at 100 mM, inhibited the uptake of exogenous PAF but did not alter the distribution of PAF metabolites in the clonal macrophage cell line. The effect ethanol has on PAF synthesis appears dependent on the activation state of the cell. Two situations were identified in this study: 1) one linked to phagocytosis or cell stimulation which is inhibited by ethanol, and 2) PAF synthesis which is not dependent on overt cell activation that is enhanced by ethanol.
使用克隆巨噬细胞系研究了乙醇对酵母聚糖吞噬作用和血小板活化因子（PAF）合成的影响。PAF被鉴定为1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱，是一种由多种细胞类型产生的生物活性醚磷脂。PAF还可激活多种炎症细胞，包括产生PAF的细胞。20至100 mM的乙醇抑制未调理酵母聚糖的吞噬作用，并同时降低受刺激巨噬细胞中PAF的浓度。相反，在未用酵母聚糖刺激的细胞中，乙醇增加了可回收的PAF。100 mM的乙醇抑制外源性PAF的摄取，但不改变克隆巨噬细胞系中PAF代谢物的分布。乙醇对PAF合成的影响似乎取决于细胞的激活状态。本研究确定了两种情况：1）一种与吞噬作用或细胞刺激有关，乙醇可抑制这种作用；2）PAF合成不依赖于明显的细胞活化，乙醇可增强这种合成。
