Marchand J C, Lavoinne A, Giroz M, Matray F
Biochimie. 1979;61(11-12):1273-82. doi: 10.1016/s0300-9084(80)80286-0.
The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 per cent). Cyclic AMP was increased (+50 per cent) and the intracellular inorganic phosphate decreased (-22 per cent). These changes were accompaned by a decrease of glycogenolysis, glucose consumption and lactate production. Measurement of glycolytic intermediates showed decreased concentrations of fructose 1,6-bis-phosphate and 3-phosphoglycerate proportional to the increase in ATP concentration. The near-equilibrium of the glyceraldehyde 3-phosphate dehydrogenase-phosphoglycerate kinase system was not modified by adenosine. The decrease of the NAD+/NADH ratio along with the increase of the ATP/ADP X PO4 ratio explains the decrease of 3-phosphoglycerate. The decrease in glucose consumption can be explained by the cross over at the phosphofructokinase stage with the decrease of fructose 1,6-bisphosphate. The major part of adenosine was deaminated as indicated by an increase in the production of ammonia and urea. The effects of inosine, or adenosine along with an inhibitor of adenosine deaminase (pentostatin) suggest that adenosine acts on the glucose consumption through adenylic nucleotides. However the increase of the adenylic nucleotide level cannot totally explain the other metabolic changes: decrease of the NAD+/NADH cytoplasmic ratio, constancy of this ratio in mitochondria, decrease of gluconeogenesis from lactate. A direct action of adenosine can therefore be expected.
对分离后的喂食大鼠肝细胞的能量代谢进行了腺苷作用的测试。将细胞在含有葡萄糖（5 mM）和腺苷（1 mM）的缓冲盐溶液培养基中于37℃孵育30分钟。这使腺苷酸核苷酸ATP的浓度增加了57%，ADP增加了39%。环磷酸腺苷增加了50%，细胞内无机磷酸盐减少了22%。这些变化伴随着糖原分解、葡萄糖消耗和乳酸生成的减少。糖酵解中间产物的测量显示，1,6 - 二磷酸果糖和3 - 磷酸甘油酸的浓度降低，与ATP浓度的增加成比例。腺苷未改变甘油醛 - 3 - 磷酸脱氢酶 - 磷酸甘油酸激酶系统的接近平衡状态。NAD⁺/NADH比值的降低以及ATP/ADP×PO₄比值的增加解释了3 - 磷酸甘油酸的减少。葡萄糖消耗的减少可以通过磷酸果糖激酶阶段果糖1,6 - 二磷酸减少的交叉作用来解释。如氨和尿素生成的增加所示，大部分腺苷被脱氨。肌苷或腺苷与腺苷脱氨酶抑制剂（喷司他丁）共同作用的结果表明，腺苷通过腺苷酸核苷酸作用于葡萄糖消耗。然而，腺苷酸核苷酸水平的增加并不能完全解释其他代谢变化：细胞质中NAD⁺/NADH比值的降低、线粒体中该比值的恒定、乳酸糖异生的减少。因此，可以预期腺苷有直接作用。
