Center of Excellence on Plant Sciences (CEPLAS), Institute of Plant Biochemistry, Heinrich-Heine-University, Düsseldorf, Germany.
Plant Biol (Stuttg). 2013 Jul;15(4):676-85. doi: 10.1111/j.1438-8677.2012.00690.x. Epub 2012 Nov 30.
Photorespiration is an essential prerequisite for all autotrophic organisms performing oxygenic photosynthesis. In contrast to the well-characterised enzymes accomplishing photorespiratory metabolism, current knowledge on the involved transport processes and the respective proteins is still quite limited. In this review, we focus on the status quo of translocators involved in photorespiratory metabolism. Although the transport of some of the photorespiratory intermediates could be characterised biochemically, using isolated organelles, the genes encoding these transporters have to date not been identified in most cases. Here, we describe the postulated transport processes, present information on established or hypothetical photorespiratory transporters, depict strategies on how to identify the transport proteins on the molecular level and, finally, discuss strategies for how to find the remaining candidates.
光合作用是所有进行需氧光合作用的自养生物的必要前提。与完成光合作用的代谢的特征明确的酶不同，目前关于涉及的转运过程和相应的蛋白质的知识仍然相当有限。在这篇综述中，我们专注于参与光合作用的代谢的转运蛋白的现状。虽然一些光合作用的中间产物的运输可以通过使用分离的细胞器进行生化特征来描述，但迄今为止，在大多数情况下，还没有鉴定出编码这些转运蛋白的基因。在这里，我们描述了假定的转运过程，介绍了已建立或假设的光合作用转运蛋白的信息，描述了如何在分子水平上鉴定转运蛋白的策略，最后讨论了如何找到其余候选者的策略。
