Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
J Surg Res. 2013 Jul;183(1):296-303. doi: 10.1016/j.jss.2012.11.009. Epub 2012 Nov 24.
Intrauterine adhesions (IUA) are associated with secondary amenorrhea, infertility, and recurrent pregnancy loss. An IUA animal model would contribute to research on the pathogenesis and pathologic changes of IUA and the exploration of new treatments.
Eighty female rabbits were randomly divided into five groups: mechanical injury (16), infectious injury (16), dual injury (16), experimental control (16), and normal (16). Three methods were applied to establish the model: uterine curettage, uterine cavity left alone, lipopolysaccharide surgical suture in place for 48 h, and suture retention for 48 h after curettage. A sterile surgical suture was left in the uterine cavity for 48 h in the experimental control group. Histologic changes were monitored at 0, 24, 48, and 72 h and 7, 14, and 28 d after operation.
The experiments revealed that endometrium injured by simple curettage or infection could be repaired. Although endometrial regeneration was severely impaired by dual injury, the ratio of the area with endometrial stromal fibrosis to total endometrial area significantly increased (P < 0.01), and the number of endometrial glands was significantly reduced (P < 0.01).
The method of dual injury can establish a stable rabbit IUA model.
宫腔粘连（IUA）与继发性闭经、不孕和反复妊娠丢失有关。IUA 动物模型有助于研究 IUA 的发病机制和病理变化，以及探索新的治疗方法。
80 只雌性兔子随机分为五组：机械损伤组（16 只）、感染损伤组（16 只）、双重损伤组（16 只）、实验对照组（16 只）和正常对照组（16 只）。采用三种方法建立模型：子宫刮宫术、宫腔不处理、48 小时放置脂多糖手术缝线和刮宫后 48 小时保留缝线。实验对照组在宫腔内留置无菌手术缝线 48 小时。术后 0、24、48 和 72 小时以及 7、14 和 28 天监测组织学变化。
实验表明，单纯刮宫或感染损伤的子宫内膜可以修复。虽然双重损伤严重损害了子宫内膜的再生，但子宫内膜基质纤维化面积与总子宫内膜面积的比例显著增加（P < 0.01），子宫内膜腺数量显著减少（P < 0.01）。
双重损伤的方法可以建立稳定的兔 IUA 模型。
