Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University / National Research Center for Assisted Reproductive Technology and Reproductive Genetics / MOE Key Laboratory of Reproductive Endocrinology (Shandong University) / Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, China.
Center for Reproductive Medicine, the Fifth People's Hospital of Jinan, Jinan 250021, China.
J Zhejiang Univ Sci B. 2018 May;19(5):383-389. doi: 10.1631/jzus.B1700086.
The pathogenesis and therapeutic treatment of intrauterine adhesions (IUAs) remain unsolved, highlighting the need for stable and effective experimental animal models. In this study, uterine electrocoagulation of twenty-one female New Zealand White rabbits was carried out to establish an IUA model. As rabbits have two completely separate uterine horns, each rabbit had its own internal control: one uterine horn was given an electrothermal injury (Group A, n=21), and the contralateral uterine horn received no treatment and served as the control (Group B, n=21). The endometrial morphology, number of endometrial glands, area of endometrial fibrosis, and number of implanted fetuses were compared between the two groups. In Group A, the numbers of endometrial glands on Days 7 and 14 and the number of implanted fetuses were significantly lower than those in Group B (P<0.05, P<0.05, and P<0.01, respectively), while the ratio of the area with endometrial stromal fibrosis to the total endometrial area was significantly increased (P<0.01). These results suggest that this method of electrothermal injury is effective for the establishment of a rabbit IUA model between 7 and 14 d after surgery.
宫腔粘连（IUAs）的发病机制和治疗方法仍未得到解决，这凸显了建立稳定有效的实验动物模型的必要性。在这项研究中，对 21 只雌性新西兰白兔进行了子宫电凝，以建立 IUA 模型。由于兔子有两个完全独立的子宫角，每只兔子都有自己的内部对照：一侧子宫角接受电热损伤（A 组，n=21），对侧子宫角未接受治疗作为对照（B 组，n=21）。比较两组子宫内膜形态、子宫内膜腺体数量、子宫内膜纤维化面积和植入胎儿数量。A 组在第 7 天和第 14 天的子宫内膜腺体数量以及植入胎儿数量明显低于 B 组（P<0.05，P<0.05 和 P<0.01），而子宫内膜间质纤维化面积与总子宫内膜面积的比值明显增加（P<0.01）。这些结果表明，这种电热损伤方法在手术后 7 至 14 天之间有效建立了兔 IUA 模型。
