Department of Medical Biochemistry, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai - 600113, Tamil Nadu, India.
Asian Pac J Trop Med. 2012 Dec;5(12):949-55. doi: 10.1016/S1995-7645(12)60180-1.
To explore the effect of alcoholic extract of Bacopa monniera (AEBM) on high cholesterol diet-induced rats.
The shade-dried and coarsely powdered whole plant material (Bacopa monniera) was extracted with 90% ethanol, finally filtered and dried in vacuum pump. The experimental rats were divided into 4 groups: control (group-I), Rats fed with hypercholesterolemic diet (HCD) for 45 days [4% cholesterol (w/w) and 1% cholic acid], Rats fed with HCD for 45 days+AEBM (40mg/kg, body weight/day orally) for last 30 days (group-III) and AEBM alone (group-IV). Blood and tissues (Aorta) were removed to ice cold containers for various biochemical and histological analysis.
AEBM treatment significantly decreased the levels of TC, TG, PL, LDL, VLDL, atherogenic index, LDL/HDL ratio, and TC/HDL ratio but significantly increased the level of HDL when compared to HCD induced rats. Activities on liver antioxidant status (SOD, CAT, GPx, GR, GST) were significantly raised with concomitant reduction in the level of LPO were obtained in AEBM treated rats when compared to HCD rats. Treatment with AEBM significantly lowered the activity of SGOT, LDH and CPK. Histopathology of aorta of cholesterol fed rat showed intimal thickening and foam cell deposition were noted.
These results suggests that AEBM extended protection against various biochemical changes and aortic pathology in hypercholesterolemic rats. Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated hypercholesterolemia.
探索益智（Bacopa monniera）醇提物对高胆固醇饮食诱导大鼠的影响。
将阴干并粗粉碎的全植物材料（益智）用 90%乙醇提取，最后过滤并在真空泵中干燥。将实验大鼠分为 4 组：对照组（I 组）、45 天高胆固醇饮食喂养的大鼠[4%胆固醇（w/w）和 1%胆酸]、45 天高胆固醇饮食喂养+AEBM（40mg/kg，体重/天口服）最后 30 天（III 组）和单独的 AEBM（IV 组）。将血液和组织（主动脉）取出到冰冷的容器中，进行各种生化和组织学分析。
与 HCD 诱导的大鼠相比，AEBM 治疗可显著降低 TC、TG、PL、LDL、VLDL、致动脉粥样硬化指数、LDL/HDL 比值和 TC/HDL 比值，而显著增加 HDL 水平。与 HCD 大鼠相比，AEBM 治疗大鼠的肝抗氧化状态（SOD、CAT、GPx、GR、GST）活性显著升高，同时 LPO 水平降低。AEBM 治疗可显著降低 SGOT、LDH 和 CPK 的活性。胆固醇喂养大鼠主动脉的组织病理学显示内膜增厚和泡沫细胞沉积。
这些结果表明，AEBM 对高胆固醇血症大鼠的各种生化变化和主动脉病理学提供了保护作用。因此，该植物可能对治疗与高胆固醇血症相关的临床病症有用。
