School of Agriculture and Food Sciences, The University of Queensland, Queensland, Australia.
Theriogenology. 2013 Feb;79(3):417-22. doi: 10.1016/j.theriogenology.2012.10.006. Epub 2012 Nov 27.
Bovine oocytes that stain with brilliant cresyl blue (BCB) have a relatively higher developmental competence. The aim of the present study was to investigate the relationships among BCB staining, lipid content, and active mitochondria. Bovine oocytes (N = 133) with at least three layers of cumulus cells were segregated as BCB retained (BCB+) or metabolized (BCB-) and then stained for active mitochondria (Mitotracker Red) and lipid (Bodipy), with analysis by confocal microscopy. The BCB+ oocytes (N = 45) contained approximately 26% more cytoplasmic lipid than BCB- oocytes (N = 26-27; P < 0.05). Staining for active mitochondria did not differ between the groups. In BCB- oocytes but not BCB+ oocytes, lipid content correlated with active mitochondrial staining (r = 0.48; P < 0.05). Diameter correlated with lipid content for BCB+ oocytes (r = 0.46; P < 0.05), but not for BCB- oocytes (r = 0.16; P > 0.05). Irrespective of BCB staining, both lipid and active mitochondrial content correlated with diameter. In conclusion, the higher lipid content of BCB+ bovine oocytes might provide a cellular and functional basis for their greater developmental competence.
用亮焦油紫（BCB）染色的牛卵母细胞具有相对较高的发育能力。本研究的目的是研究 BCB 染色、脂质含量和活性线粒体之间的关系。将至少有三层卵丘细胞的牛卵母细胞（N = 133）分为 BCB 保留（BCB+）或代谢（BCB-），然后用活性线粒体（Mitotracker Red）和脂质（Bodipy）染色，通过共聚焦显微镜进行分析。BCB+卵母细胞（N = 45）的细胞质脂质含量比 BCB-卵母细胞（N = 26-27）多约 26%（P < 0.05）。两组间活性线粒体染色无差异。在 BCB-卵母细胞中，但不在 BCB+卵母细胞中，脂质含量与活性线粒体染色呈正相关（r = 0.48；P < 0.05）。BCB+卵母细胞的直径与脂质含量呈正相关（r = 0.46；P < 0.05），但 BCB-卵母细胞无此相关性（r = 0.16；P > 0.05）。无论 BCB 染色如何，脂质和活性线粒体含量均与直径相关。总之，BCB+牛卵母细胞中较高的脂质含量可能为其更高的发育能力提供了细胞和功能基础。
