Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Seattle, WA 98112, USA.
Mol Cell Endocrinol. 2013 Feb 5;366(1):38-52. doi: 10.1016/j.mce.2012.11.015. Epub 2012 Nov 27.
Follicle-stimulating hormone (Fsh) function in fishes is poorly understood. This study aimed to reveal Fsh-regulated genes in coho salmon previtellogenic ovarian follicles in vitro. Four suppression subtractive hybridization libraries were generated with RNA isolated from Fsh-treated and control follicles or follicle cell-enriched tissue fractions. Fsh induced steroidogenesis and dynamically upregulated several genes predominantly expressed in follicle cells, including WAP domain-containing protease, connexin 34.3, clusterin (clu1, clu2), fibronectin, wilms tumor 2-like, and influenza virus NS1A-binding protein a. Genes downregulated by Fsh included connective tissue growth factor, alcohol dehydrogenase 8-like, and serine/threonine-protein kinase pim-1. This study demonstrates for the first time in fishes that Fsh influences the expression of a unique suite of ovarian genes involved in processes like cell communication, survival and differentiation, and extracellular matrix remodeling. Collectively, these findings suggest that Fsh and/or steroids induce differentiation of granulosa cells and remodeling of the follicle in preparation for onset of vitellogenesis.
鱼类中促卵泡激素（Fsh）的功能尚未完全了解。本研究旨在体外揭示三文鱼始基卵泡中 Fsh 调节的基因。从 Fsh 处理和对照卵泡或卵泡细胞富集组织部分分离的 RNA 生成了四个抑制性消减杂交文库。Fsh 诱导了类固醇生成，并动态地上调了几个主要在卵泡细胞中表达的基因，包括 WAP 结构域蛋白酶、连接蛋白 34.3、簇蛋白（clu1、clu2）、纤连蛋白、Wilms 肿瘤 2 样蛋白和流感病毒 NS1A 结合蛋白 a。Fsh 下调的基因包括结缔组织生长因子、醇脱氢酶 8 样和丝氨酸/苏氨酸蛋白激酶 pim-1。本研究首次在鱼类中证明，Fsh 影响一系列独特的卵巢基因的表达，这些基因参与细胞通讯、存活和分化以及细胞外基质重塑等过程。总的来说，这些发现表明 Fsh 和/或类固醇诱导颗粒细胞的分化和卵泡的重塑，为卵黄生成的开始做准备。
