Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Prague, Czech Republic.
N Biotechnol. 2013 Mar 25;30(3):314-8. doi: 10.1016/j.nbt.2012.11.009. Epub 2012 Nov 29.
It is generally assumed that human endogenous retroviral elements (HERVs) belong to the class of genomic repetitive nucleotide sequences often called 'junk DNA'. These elements were categorized to families, and members of some of these families (e.g. HERV-H, HERV-W and HERV-K) were shown to be transcribed. These transcriptions were associated with several severe diseases such as mental disorders, AIDS, autoimmune diseases and cancer. In this review we discuss several bioinformatics strategies for genome-wide scan of HERVs transcription using high-throughput RNA sequencing on several platforms. We show that many more HERVs than previously described are transcribed to various levels and we discuss possible implications of these transcriptions.
一般认为，人类内源性逆转录病毒元件（HERV）属于基因组重复核苷酸序列类别，通常被称为“垃圾 DNA”。这些元件被分类为家族，其中一些家族的成员（例如 HERV-H、HERV-W 和 HERV-K）被证明可以转录。这些转录与多种严重疾病有关，如精神障碍、艾滋病、自身免疫性疾病和癌症。在这篇综述中，我们讨论了几种使用多种平台上的高通量 RNA 测序进行全基因组 HERV 转录扫描的生物信息学策略。我们表明，有比以前描述的更多的 HERV 被转录到不同的水平，我们讨论了这些转录的可能意义。
