Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark.
Res Microbiol. 2013 Apr;164(3):205-10. doi: 10.1016/j.resmic.2012.11.005. Epub 2012 Nov 28.
The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
Clp ATPases 家族在细菌生理学中起着重要作用。在这里，我们描述了一个新发现的质粒编码的 ClpK 蛋白的遗传基因座，该蛋白在热激期间保护肺炎克雷伯菌细胞。我们证明 clpK 基因位于一个多顺反子操纵子中，可变的下游基因含量与不同分离株的耐热表型相关。ClpK 由一个多功能转录单元编码，该单元具有 ClpP 依赖性和非依赖性活性。值得注意的是，我们的数据表明，ClpP 对 ClpK 单独发挥的热保护作用是必不可少的，这表明 ClpK 是 ClpP 相互作用的 Clp ATPases 家族的新成员。
