Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
Exp Parasitol. 2013 Feb;133(2):156-61. doi: 10.1016/j.exppara.2012.11.008. Epub 2012 Nov 29.
There are few studies on human ocular leishmaniasis found in the literature. The purpose of this study was to describe experimental ocular leishmaniasis, caused by Leishmania amazonensis evaluating two different infection routes: intravitreal and instillation in C57BL/10 and BALB/c mice. In this work all animals presented low anti-Leishmania IgM and IgG titers regardless of the infection route or mouse strain. The histopathological eye analysis showed that the mice inoculated by the intravitreal route developed more severe lesions, presenting parasites in the anterior region of the eye 60 days after infection. The C57BL/10 mice presented cells containing parasitophorous vacuoles associated with pigmented cells and inflammatory infiltrate, which included mast cells. Ninety days after infection no parasites could be found in either mouse strain, which led us to hypothesize that parasites had been eliminated. In this context, we show that both intravitreal and instillation routes were effective in promoting ocular leishmaniasis infections in C57BL/10 and BALB/c mice. There were no differences in the parasite infection between the two mouse models and it mimicked the ocular lesions described in symptomatic dogs in endemic areas of visceral leishmaniasis.
目前文献中关于人类眼利什曼病的研究甚少。本研究旨在描述由 Leishmania amazonensis 引起的实验性眼利什曼病，评估两种不同的感染途径：眼内注射和滴注到 C57BL/10 和 BALB/c 小鼠中。在这项工作中，无论感染途径或小鼠品系如何，所有动物的抗 Leishmania IgM 和 IgG 滴度均较低。眼部组织病理学分析表明，通过眼内注射途径接种的小鼠在感染后 60 天眼部出现更严重的病变，在前眼部区域发现寄生虫。C57BL/10 小鼠中含有寄生空泡的细胞与色素细胞和炎症浸润有关，其中包括肥大细胞。感染 90 天后，两种小鼠品系均未发现寄生虫，这使我们假设寄生虫已被清除。在这种情况下，我们表明眼内注射和滴注途径均可有效促进 C57BL/10 和 BALB/c 小鼠的眼利什曼病感染。两种小鼠模型之间的寄生虫感染没有差异，并且模拟了内脏利什曼病流行地区有症状犬中描述的眼部病变。
