Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, 229 North TaiBai Road, Xi'an City 710069, China.
Food Chem Toxicol. 2013 May;55:234-40. doi: 10.1016/j.fct.2012.11.022. Epub 2012 Nov 28.
The aim of the present study was to investigate the antioxidant and hepatotective effects of Schisandra chinensis pollen extract (SCPE) on CCl4-induced acute liver damage in mice. Total phenolic content, total flavonoid content, individual phenolic compounds and antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, chelating activity, and reducing power assay) were determined. In vivo study, SCPE (10, 20 and 40g/kg) administered daily orally for 42days prior to CCl4-intoxicated. Our results showed that SCPE had high total phenolic content (53.74±1.21mg GAE/g), total flavonoid content (38.29±0.91mg Rutin/g), quercetin and hesperetin may be the major contributor to strong antioxidant activities. Moreover, SCPE significantly prevented the increase in serum ALT and AST level in acute liver damage induced by CCl4, decreased the extent of malondialdehyde (MDA) formation in liver and elevated the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver. The results indicated that SCPE has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl4, and have been supported by the evaluation of liver histopathology in mice. The hepatoprotective effect may be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.
本研究旨在探讨五味子花粉提取物（SCPE）对四氯化碳（CCl4）诱导的小鼠急性肝损伤的抗氧化和保肝作用。测定了总酚含量、总黄酮含量、单体酚类化合物和抗氧化活性（1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、螯合活性和还原力测定）。在体内研究中，SCPE（10、20 和 40g/kg）在 CCl4 中毒前每天口服给药 42 天。结果表明，SCPE 具有较高的总酚含量（53.74±1.21mg GAE/g）、总黄酮含量（38.29±0.91mg 芦丁/g），槲皮素和橙皮苷可能是其具有较强抗氧化活性的主要原因。此外，SCPE 可显著防止 CCl4 诱导的急性肝损伤血清 ALT 和 AST 水平升高，降低肝丙二醛（MDA）形成程度，提高肝中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。结果表明，SCPE 具有较强的抗氧化活性，对 CCl4 诱导的急性肝毒性具有显著的保护作用，这一作用得到了小鼠肝组织病理学评价的支持。其保肝作用可能与其清除自由基、提高抗氧化活性和抑制脂质过氧化有关。
