Souid Aymen, Giambastiani Lucia, Castagna Antonella, Santin Marco, Vivarelli Fabio, Canistro Donatella, Morosini Camilla, Paolini Moreno, Franchi Paola, Lucarini Marco, Raffaelli Andrea, Giorgetti Lucia, Ranieri Annamaria, Longo Vincenzo, Pozzo Luisa, Vornoli Andrea
Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
Institute of Agricultural Biology and Biotechnology-National Research Council (IBBA-CNR), Via Moruzzi 1, 56124 Pisa, Italy.
Antioxidants (Basel). 2024 May 12;13(5):596. doi: 10.3390/antiox13050596.
Halophyte species represent valuable reservoirs of natural antioxidants, and, among these, stands out as a promising edible plant. In this study, young and old leaves were compared for the content of bioactive compounds and antioxidant activity to assess changes in different growth phases; then, the potential protective effects against low-dose CCl-induced toxicant-associated fatty liver disease (TAFLD) were investigated by administering an aqueous suspension of young leaves to rats daily for two weeks. Quantification of total and individual phenolic compounds and in vitro antioxidant activity assays (DPPH, FRAP, and ORAC) showed the highest values in young leaves compared to mature ones. Salicornia treatment mitigated CCl-induced hepatic oxidative stress, reducing lipid peroxidation and protein carbonyl levels, and preserving the decrease in glutathione levels. Electronic paramagnetic resonance (EPR) spectroscopy confirmed these results in the liver and evidenced free radicals increase prevention in the brain. Salicornia treatment also attenuated enzymatic disruptions in the liver's drug metabolizing system and Nrf2-dependent antioxidant enzymes. Furthermore, histopathological examination revealed reduced hepatic lipid accumulation and inflammation. Overall, this study highlights Salicornia's potential as a source of bioactive compounds with effective hepatoprotective properties capable to prevent TAFLD.
盐生植物物种是天然抗氧化剂的宝贵来源，其中，[盐角草属植物]作为一种很有前景的可食用植物脱颖而出。在本研究中，比较了盐角草属植物幼叶和老叶中生物活性化合物的含量和抗氧化活性，以评估不同生长阶段的变化；然后，通过每天给大鼠喂食幼叶水悬浮液，持续两周，研究其对低剂量四氯化碳诱导的毒物相关性脂肪性肝病（TAFLD）的潜在保护作用。总酚和单个酚类化合物的定量分析以及体外抗氧化活性测定（DPPH、FRAP和ORAC）显示，与成熟叶相比，幼叶中的含量最高。盐角草属植物处理减轻了四氯化碳诱导的肝脏氧化应激，降低了脂质过氧化和蛋白质羰基水平，并维持了谷胱甘肽水平的下降。电子顺磁共振（EPR）光谱证实了肝脏中的这些结果，并证明了大脑中自由基增加的预防作用。盐角草属植物处理还减轻了肝脏药物代谢系统和Nrf2依赖性抗氧化酶的酶促破坏。此外，组织病理学检查显示肝脏脂质积累和炎症减少。总体而言，本研究突出了盐角草属植物作为具有有效肝脏保护特性、能够预防TAFLD的生物活性化合物来源的潜力。
