Water and Environmental Science and Technology Department, Environmental Engineering Group, University of Cantabria, 39005 Santander, Cantabria, Spain.
Bioresour Technol. 2013 Jan;128:324-9. doi: 10.1016/j.biortech.2012.10.114. Epub 2012 Nov 2.
High pH-induced flocculation-sedimentation of Scenedesmus obliquus and Chlorella vulgaris was studied with the objective of improving the efficiency of microalgae harvesting, since it is one of the most expensive steps of production of microalgae. Desired pH values were achieved by addition of NaOH and Ca(OH)(2). Growth rate and lipid productivity in fresh media prepared with tap water and with analytical-grade water, and in reused media prepared with culture centrifuged supernatant and the supernatant from high pH-induced flocculation-sedimentation were compared. Since the growth rates for reused media were about 1.7 times higher than in fresh media, and the lipid productivities were about 25 and 26 mg L(-1) d(-1) in flocculated and centrifuged reused media respectively, medium reuse is a suitable method of saving water and nutrients.
采用添加 NaOH 和 Ca(OH)2 的方法来调节 pH 值，研究了 pH 值升高对斜生栅藻和普通小球藻絮凝-沉淀的影响，以期提高微藻收获的效率，因为这是微藻生产中最昂贵的步骤之一。以自来水和分析纯级水为基础培养液，比较了在其中培养的藻的生长率和产油率，以及以培养后的离心上清液和 pH 值升高诱导的絮凝-沉淀后的上清液为基础培养液在重复使用时的藻的生长率和产油率。由于重复使用培养基的生长率比新鲜培养基高约 1.7 倍，而在絮凝和离心的重复使用培养基中，产油率分别约为 25 和 26mg·L-1·d-1，因此，培养基的重复使用是一种节约水和营养物质的合适方法。
