Departament d'Enginyeria Química, Biològica i Ambiental, Escola d'Enginyeria (EE), Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Departament d'Enginyeria Química, Biològica i Ambiental, Escola d'Enginyeria (EE), Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Bioresour Technol. 2016 Sep;216:211-8. doi: 10.1016/j.biortech.2016.05.063. Epub 2016 May 21.
Combined effect of ferric chloride and pH on Chlorella vulgaris growth and flocculation were optimized using DoE. Afterwards, an integrated bioprocess for microalgae cultivation and harvesting conceived as a sole step was run in continuous operation mode. Microalgae concentration in a 2L-photobioreactor was about 0.5gL(-1) and the efficiency of flocculation in the coupled sedimentation tank was about 95%. Dewatered microalgae reached a biomass concentrations increase about 50-fold, whereas it was only about 0.02gL(-1) in the clarified medium. Then, the reuse of the clarified medium recovered was further evaluated. The clarified medium was reused without any further nutrient supplementation, whereas a second round of medium reuse was performed after supplementation of main nutrients (phosphate-sulfate-nitrate), micronutrients and ferric chloride. The medium reuse strategy did not affect cell growth and flocculation. Consequently, the reuse of medium reduces the nutrients requirements and the demand for water, and therefore the production costs should be reduced accordingly.
采用 DOE 对三氯化铁和 pH 值对小球藻生长和絮凝的联合作用进行了优化。之后，以连续操作模式运行了一个设计为单一步骤的微藻培养和收获的集成生物工艺。在 2L 光生物反应器中，微藻浓度约为 0.5gL(-1)，在耦合沉降池中絮凝效率约为 95%。脱水后的微藻生物量浓度增加了约 50 倍，而在澄清培养基中仅约为 0.02gL(-1)。然后，进一步评估了回收澄清培养基的再利用。在不添加任何额外营养物质的情况下，重复使用澄清培养基，而在补充主要营养物质（磷酸盐-硫酸盐-硝酸盐）、微量元素和三氯化铁后，进行第二轮培养基再利用。培养基再利用策略不会影响细胞生长和絮凝。因此，培养基的再利用减少了对营养物质和水的需求，从而降低了生产成本。
