Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
Bioresour Technol. 2013 Jan;128:423-30. doi: 10.1016/j.biortech.2012.10.116. Epub 2012 Nov 2.
The adsorptive removal of Cr(VI) by alginate beads containing Cr(VI)-adapted Acinetobacter junii, Escherichia coli and Bacillus subtilis in batch and continuous packed bed column reactors was investigated. Under optimized conditions (pH 3.0; contact time, 180 min; 30 °C; initial Cr(VI) concentration of 100 mg/L), 65.86 mg/g adsorption capacity was recorded in the batch study. When an adsorbent dosage of 1g/L, a flow rate of 5 mL/min, a bed height of 20 cm, an initial Cr(VI) concentration of 300 mg/L was employed, a capacity of 657 mg/g was noted for the continuous column assay. The batch sorption data followed the Langmuir isotherm and pseudo second order kinetics. Five sorption/desorption cycles yielded 100%, 99.63%, 95.31%, 80.7% and 74.22% regeneration, respectively. Cr(VI) adsorption studies using spiked ground water, freshwater and domestic wastewater in a packed bed reactor demonstrated Cr(VI) removals of 64.8%, 55.08%, 56.86% respectively. Cr(VI) sorption on immobilized bacteria was confirmed with Fourier-transform infrared and Energy dispersive X-ray spectroscopy.
采用含 Cr(VI)-适应的不动杆菌 junii、大肠杆菌和枯草芽孢杆菌的海藻酸钠珠粒在批量和连续填充床柱反应器中吸附去除 Cr(VI)。在优化条件下（pH 3.0；接触时间 180 分钟；30°C；初始 Cr(VI)浓度为 100mg/L），在批量研究中记录到 65.86mg/g 的吸附容量。当采用 1g/L 的吸附剂剂量、5mL/min 的流速、20cm 的床层高度和 300mg/L 的初始 Cr(VI)浓度时，连续柱试验的容量为 657mg/g。批量吸附数据遵循 Langmuir 等温线和伪二级动力学。五次吸附/解吸循环分别产生 100%、99.63%、95.31%、80.7%和 74.22%的再生。在填充床反应器中使用加标地下水、淡水和生活污水进行 Cr(VI)吸附研究，分别实现了 64.8%、55.08%和 56.86%的 Cr(VI)去除率。通过傅里叶变换红外和能量色散 X 射线光谱证实了固定化细菌对 Cr(VI)的吸附。
