College of Tea & Food Science and Technology, Anhui Agricultural University, Key Laboratory of Analysis and Detection Technology for Food Safety, Hefei, Anhui 230036, China.
Colloids Surf B Biointerfaces. 2013 Mar 1;103:107-13. doi: 10.1016/j.colsurfb.2012.10.013. Epub 2012 Oct 23.
Fe(3)O(4) modified by chitosan (CTS) and Staphylococcus aureus enterotoxin A (SEA) antiserum immunomagnetic beads (Fe(3)O(4)-CTS-SEA-IB) that targeted enrich SEA were prepared in aqueous solution and using CTS and SEA antiserum as surface modification stabilizer. Recognition experiments of the prepared Fe(3)O(4)-CTS-SEA-IB on SEA were conducted to determine adsorption capacity and recognition specificity. The results showed that the SEA antiserum were prepared and successfully conjugated onto Fe(3)O(4)-modificated CTS magnetic beads. The Fe(3)O(4)-CTS-SEA immunomagnetic beads displayed a high adsorption capacity and recognition specificity for SEA, and the adsorption quantity could reach 6.48 × 10(-3) μmol/g. The specificity evaluation results showed that the Fe(3)O(4)-CTS-SEA immunomagnetic beads had high enrichment and affinity property for SEA compared to SEB (Staphylococcus aureus enterotoxin B) and SPA (Staphylococcus aureus protein A).
用壳聚糖（CTS）和金黄色葡萄球菌肠毒素 A（SEA）抗血清免疫磁珠修饰的 Fe(3)O(4)（Fe(3)O(4)-CTS-SEA-IB），以靶向富集 SEA，在水溶液中制备，并用 CTS 和 SEA 抗血清作为表面修饰稳定剂。对制备的 Fe(3)O(4)-CTS-SEA-IB 对 SEA 的识别实验进行了检测，以确定吸附容量和识别特异性。结果表明，SEA 抗血清被制备并成功地连接到 Fe(3)O(4)-修饰的 CTS 磁性珠上。Fe(3)O(4)-CTS-SEA 免疫磁珠对 SEA 表现出高吸附容量和识别特异性，吸附量可达 6.48×10(-3) μmol/g。特异性评价结果表明，与 SEB（金黄色葡萄球菌肠毒素 B）和 SPA（金黄色葡萄球菌蛋白 A）相比，Fe(3)O(4)-CTS-SEA 免疫磁珠对 SEA 具有更高的富集和亲和力。
