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来自原核和真核光合微生物的金纳米粒子——合成及其在生物标记中的应用比较研究。

Gold nanoparticles from pro and eukaryotic photosynthetic microorganisms--comparative studies on synthesis and its application on biolabelling.

机构信息

Division of Microbial Biodiversity and Bioenergy, Department of Microbiology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.

出版信息

Colloids Surf B Biointerfaces. 2013 Mar 1;103:166-73. doi: 10.1016/j.colsurfb.2012.10.014. Epub 2012 Oct 17.

Abstract

Today, a material science focuses on the nanoparticles synthesis in general and synthesizing them by biological entity in particular for their marvel production and its remarkable property. In this present study, synthesis of gold nanoparticles using photosynthetic microorganisms such as Coelastrella sp. (eukaryotes) and Phormidium sp. (prokaryotes) were reacted with Chloroauric acid (HAuCl(4)) and bioaccumulation was assessed. Various techniques were adopted for characterization of nanoparticles and compared. It was found to be 25 nm sized nanotriangles and 30 nm sized spherical shaped nanoparticles were synthesized by prokaryotic and eukaryotic microorganisms respectively by TEM analysis. Biogenic gold nanoparticles have potent antioxidant property and the interaction of gold nanoparticles with DNA was evaluated that biogenic nanoparticles were actively bound to DNA in increased concentration. It was revealed that biogenic nanoparticles have wide range of applications depends on the biological entity used. Selection of suitable biological entity is very much important for the production of nanoparticles with desirable shapes and size for the biomedical applications.

摘要

如今,材料科学主要关注纳米粒子的合成,特别是通过生物实体来合成纳米粒子,以获得其惊人的产量和显著的性能。在本研究中,使用光合微生物(如 Coelastrella sp. [真核生物]和 Phormidium sp. [原核生物])与氯金酸(HAuCl(4))反应来合成金纳米粒子,并评估其生物累积情况。采用了各种技术对纳米粒子进行了表征,并进行了比较。通过 TEM 分析发现,原核和真核微生物分别合成了 25nm 大小的纳米三角形和 30nm 大小的球形纳米粒子。生物合成的金纳米粒子具有很强的抗氧化性能,并且还评估了金纳米粒子与 DNA 的相互作用,结果表明,生物合成的纳米粒子在增加浓度时会主动与 DNA 结合。研究表明,生物合成的纳米粒子具有广泛的应用范围,这取决于所使用的生物实体。选择合适的生物实体对于生产具有理想形状和尺寸的用于生物医学应用的纳米粒子非常重要。

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