Department of Clinical Sciences, Medicine, Lund University, SE221 84, Lund, Sweden.
Nutrients. 2012 Sep 28;4(10):1367-81. doi: 10.3390/nu4101367.
The C57BL/6 mouse fed a high fat diet is a common and valuable model in experimental studies of obesity and type 2 diabetes (T2D). Different high fat diets are used and in order to determine which diet produces a model most accurately resembling human T2D, they need to be compared head-to-head.
Four different diets, the 60% high fat diet (HFD) and the 58% high fat-high sucrose Surwit diet (HFHS) and their respective controls, were compared in C57BL/6J mice using glucose tolerance tests (IVGTT) and the euglycemic clamp.
Mice fed a HFD gained more weight than HFHS fed mice despite having similar energy intake. Both high fat diet models were glucose intolerant after eight weeks. Mice fed the HFD had elevated basal insulin, which was not seen in the HFHS group. The acute insulin response (AIR) was unchanged in the HFD group, but slightly increased in the HFHS diet group. The HFHS diet group had a threefold greater total insulin secretion during the IVGTT compared to its control, while no differences were seen in the HFD group. Insulin sensitivity was decreased fourfold in the HFD group, but not in the HFHS diet group.
The HFD and HFHS diet models show differential effects on the development of insulin resistance and beta cell adaptation. These discrepancies are important to acknowledge in order to select the appropriate diet for specific studies.
C57BL/6 小鼠高脂饮食是肥胖和 2 型糖尿病（T2D）实验研究中常用且有价值的模型。不同的高脂饮食被使用，为了确定哪种饮食最能准确模拟人类 T2D，需要进行头对头比较。
在 C57BL/6J 小鼠中比较了四种不同的饮食，即 60%高脂饮食（HFD）和 58%高脂高蔗糖 Surwit 饮食（HFHS）及其各自的对照饮食，使用葡萄糖耐量试验（IVGTT）和正葡萄糖钳夹进行比较。
尽管能量摄入相似，但喂食 HFD 的小鼠比 HFHS 喂养的小鼠体重增加更多。两种高脂肪饮食模型在 8 周后均出现葡萄糖不耐受。喂食 HFD 的小鼠基础胰岛素升高，而 HFHS 组则没有。HFD 组的急性胰岛素反应（AIR）没有变化，但 HFHS 饮食组略有增加。HFHS 饮食组在 IVGTT 期间的总胰岛素分泌量是其对照饮食组的三倍，而 HFD 组则没有差异。HFD 组的胰岛素敏感性降低了四倍，但 HFHS 饮食组没有。
HFD 和 HFHS 饮食模型对胰岛素抵抗和β细胞适应性的发展有不同的影响。为了选择特定研究的合适饮食，这些差异非常重要。
