• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

棕色脂肪组织（BAT）特异性 vaspin 表达在致肥胖饮食和冷暴露后增加，并与 DNA 甲基化的急性变化相关。

Brown adipose tissue (BAT) specific vaspin expression is increased after obesogenic diets and cold exposure and linked to acute changes in DNA-methylation.

机构信息

Divisions of Endocrinology and Nephrology, University of Leipzig, Leipzig, Germany.

Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany.

出版信息

Mol Metab. 2017 Mar 28;6(6):482-493. doi: 10.1016/j.molmet.2017.03.004. eCollection 2017 Jun.

DOI:10.1016/j.molmet.2017.03.004
PMID:28580279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5444018/
Abstract

OBJECTIVE

Several studies have demonstrated anti-diabetic and anti-obesogenic properties of visceral adipose tissue-derived serine protease inhibitor (vaspin) and so evoked its potential use for treatment of obesity-related diseases. The aim of the study was to unravel physiological regulators of vaspin expression and secretion with a particular focus on its role in brown adipose tissue (BAT) biology.

METHODS

We analyzed the effects of obesogenic diets and cold exposure on vaspin expression in liver and white and brown adipose tissue (AT) and plasma levels. Vaspin expression was analyzed in isolated white and brown adipocytes during adipogenesis and in response to adrenergic stimuli. DNA-methylation within the vaspin promoter was analyzed to investigate acute epigenetic changes after cold-exposure in BAT.

RESULTS

Our results demonstrate a strong induction of vaspin mRNA and protein expression specifically in BAT of both cold-exposed and high-fat (HF) or high-sugar (HS) fed mice. While obesogenic diets also upregulated hepatic mRNA levels, cold exposure tended to increase gene expression of inguinal white adipose tissue (iWAT) depots. Concomitantly, vaspin plasma levels were decreased upon obesogenic or thermogenic triggers. Vaspin expression was increased during adipogenesis but unaffected by sympathetic activation in brown adipocytes. Analysis of vaspin promoter methylation in AT revealed lowest methylation levels in BAT, which were acutely reduced after cold exposure.

CONCLUSIONS

Our data demonstrate a novel BAT-specific regulation of gene expression upon physiological stimuli with acute epigenetic changes that may contribute to cold-induced expression in BAT. We conclude that these findings indicate functional relevance and potentially beneficial effects of vaspin in BAT function.

摘要

目的

多项研究表明内脏脂肪组织来源的丝氨酸蛋白酶抑制剂（vaspin）具有抗糖尿病和抗肥胖作用，因此激发了其在治疗肥胖相关疾病中的潜在用途。本研究旨在揭示 vaspin 表达和分泌的生理调节因子，特别关注其在棕色脂肪组织（BAT）生物学中的作用。

方法

我们分析了肥胖饮食和冷暴露对肝脏和白色及棕色脂肪组织（AT）中 vaspin 表达和血浆水平的影响。在脂肪生成过程中以及对肾上腺素能刺激的反应中，分析了分离的白色和棕色脂肪细胞中 vaspin 的表达。分析 vaspin 启动子内的 DNA 甲基化，以研究冷暴露后 BAT 中的急性表观遗传变化。

结果

我们的结果表明，冷暴露和高脂肪（HF）或高糖（HS）喂养的小鼠的 BAT 中，vaspin mRNA 和蛋白表达均得到强烈诱导。虽然肥胖饮食也上调了肝脏 mRNA 水平，但冷暴露往往会增加腹股沟白色脂肪组织（iWAT）的基因表达。同时，肥胖或产热触发会降低 vaspin 的血浆水平。vaspin 在脂肪生成过程中表达增加，但在棕色脂肪细胞中不受交感神经激活的影响。AT 中 vaspin 启动子甲基化分析显示，BAT 中的甲基化水平最低，冷暴露后急性降低。

结论

我们的数据表明，生理刺激下 BAT 中存在新型的基因表达特异性调节，伴有急性表观遗传变化，这可能有助于 BAT 中冷诱导的表达。我们得出结论，这些发现表明 vaspin 在 BAT 功能中具有功能相关性和潜在的有益作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/970962e550c2/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/cb2eefd55d20/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/b4b69fa7be64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/018a6b6b7794/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/e9fc71e20ef6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/fdcabf4ff518/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/d20bbb2c54ff/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/aa8d04713719/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/970962e550c2/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/cb2eefd55d20/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/b4b69fa7be64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/018a6b6b7794/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/e9fc71e20ef6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/fdcabf4ff518/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/d20bbb2c54ff/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/aa8d04713719/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/342d/5444018/970962e550c2/gr8.jpg

相似文献

1
Brown adipose tissue (BAT) specific vaspin expression is increased after obesogenic diets and cold exposure and linked to acute changes in DNA-methylation.棕色脂肪组织（BAT）特异性 vaspin 表达在致肥胖饮食和冷暴露后增加，并与 DNA 甲基化的急性变化相关。
Mol Metab. 2017 Mar 28;6(6):482-493. doi: 10.1016/j.molmet.2017.03.004. eCollection 2017 Jun.
2
Bone morphogenic protein 9 is a novel thermogenic hepatokine secreted in response to cold exposure.骨形态发生蛋白 9 是一种新型的冷暴露反应性产热肝细胞因子。
Metabolism. 2022 Apr;129:155139. doi: 10.1016/j.metabol.2022.155139. Epub 2022 Jan 19.
3
Cold acclimation and pioglitazone combined increase thermogenic capacity of brown and white adipose tissues but this does not translate into higher energy expenditure in mice.冷适应与吡格列酮联合使用可增加棕色和白色脂肪组织的产热能力，但这并未转化为小鼠更高的能量消耗。
Am J Physiol Endocrinol Metab. 2023 Apr 1;324(4):E358-E373. doi: 10.1152/ajpendo.00217.2022. Epub 2023 Mar 1.
4
Overexpression of Adiponectin Receptor 1 Inhibits Brown and Beige Adipose Tissue Activity in Mice.脂联素受体 1 过表达抑制小鼠棕色和米色脂肪组织活性。
Int J Mol Sci. 2021 Jan 18;22(2):906. doi: 10.3390/ijms22020906.
5
Histone proteoform analysis reveals epigenetic changes in adult mouse brown adipose tissue in response to cold stress.组蛋白蛋白质异构体分析揭示了成年小鼠棕色脂肪组织在冷应激反应中的表观遗传变化。
Epigenetics Chromatin. 2024 Apr 27;17(1):12. doi: 10.1186/s13072-024-00536-8.
6
Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing.贝拉尔迪内利-塞普先天性脂肪代谢障碍2型/含硒蛋白决定棕色脂肪组织的维持和产热编程。
Mol Metab. 2020 Jun;36:100971. doi: 10.1016/j.molmet.2020.02.014. Epub 2020 Mar 4.
7
JAK2 promotes brown adipose tissue function and is required for diet- and cold-induced thermogenesis in mice.JAK2促进棕色脂肪组织功能，是小鼠饮食诱导和寒冷诱导产热所必需的。
Diabetologia. 2016 Jan;59(1):187-196. doi: 10.1007/s00125-015-3786-2. Epub 2015 Oct 29.
8
14-3-3ζ mediates an alternative, non-thermogenic mechanism in male mice to reduce heat loss and improve cold tolerance.14-3-3ζ 在雄性小鼠中介导一种替代的、非产热的机制，减少热量损失，提高耐寒性。
Mol Metab. 2020 Nov;41:101052. doi: 10.1016/j.molmet.2020.101052. Epub 2020 Jul 12.
9
GHS-R in brown fat potentiates differential thermogenic responses under metabolic and thermal stresses.GHS-R 在棕色脂肪中发挥作用，增强代谢和热应激下的不同产热反应。
PLoS One. 2021 Apr 1;16(4):e0249420. doi: 10.1371/journal.pone.0249420. eCollection 2021.
10
Adipocyte-specific mTORC2 deficiency impairs BAT and iWAT thermogenic capacity without affecting glucose uptake and energy expenditure in cold-acclimated mice.脂肪细胞特异性 mTORC2 缺乏会损害 BAT 和 iWAT 的产热能力，而不影响冷适应小鼠的葡萄糖摄取和能量消耗。
Am J Physiol Endocrinol Metab. 2021 Nov 1;321(5):E592-E605. doi: 10.1152/ajpendo.00587.2020. Epub 2021 Sep 20.

引用本文的文献

1
Dynamic Rendition of Adipose Genes Under Epigenetic Regulation: Revealing New Mechanisms of Obesity Occurrence.表观遗传调控下脂肪基因的动态呈现：揭示肥胖发生的新机制
Curr Issues Mol Biol. 2025 Jul 11;47(7):540. doi: 10.3390/cimb47070540.
2
Cold-Induced DHRS4 Promotes Thermogenesis via Enhanced Fatty Acid β-Oxidation in Porcine Subcutaneous Adipocytes.冷诱导的DHRS4通过增强猪皮下脂肪细胞中的脂肪酸β-氧化促进产热。
Animals (Basel). 2025 Apr 22;15(9):1190. doi: 10.3390/ani15091190.
3
Exercise Alleviates Aging of Adipose Tissue through Adipokine Regulation.

本文引用的文献

1
High fat diet-induced changes of mouse hepatic transcription and enhancer activity can be reversed by subsequent weight loss.高脂肪饮食诱导的小鼠肝转录和增强子活性变化可通过随后的体重减轻得到逆转。
Sci Rep. 2017 Jan 10;7:40220. doi: 10.1038/srep40220.
2
Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice.甲状腺激素状态定义了小鼠棕色脂肪组织的活性和白色脂肪组织的褐变。
Sci Rep. 2016 Dec 12;6:38124. doi: 10.1038/srep38124.
3
Basic Residues of β-Sheet A Contribute to Heparin Binding and Activation of Vaspin (Serpin A12).
运动通过调节脂肪因子减轻脂肪组织衰老。
Metabolites. 2024 Feb 22;14(3):135. doi: 10.3390/metabo14030135.
4
Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice.雌性小鼠在高脂高糖饮食下脂肪组织和肝脏中免疫调节细胞因子的差异表达。
Front Nutr. 2023 Nov 3;10:1275160. doi: 10.3389/fnut.2023.1275160. eCollection 2023.
5
Overexpressing high levels of human vaspin limits high fat diet-induced obesity and enhances energy expenditure in a transgenic mouse.高表达人 vaspin 水平限制高脂肪饮食诱导的肥胖，并增强转基因小鼠的能量消耗。
Front Endocrinol (Lausanne). 2023 Apr 19;14:1146454. doi: 10.3389/fendo.2023.1146454. eCollection 2023.
6
, and Are Potential Regulators of Brown Adipose Tissue Development Associated with Obesity-Related Metabolic Dysfunction in Children. 和 是与儿童肥胖相关代谢功能障碍相关的棕色脂肪组织发育的潜在调节因子。
Int J Mol Sci. 2023 Feb 4;24(4):3085. doi: 10.3390/ijms24043085.
7
Myoglobin-mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humans.肌红蛋白介导的脂质转运增加了肾上腺素能对棕色和白色脂肪细胞代谢的激活，并且是人类产热脂肪细胞的标志物。
Clin Transl Med. 2022 Dec;12(12):e1108. doi: 10.1002/ctm2.1108.
8
Adipokines, Hepatokines and Myokines: Focus on Their Role and Molecular Mechanisms in Adipose Tissue Inflammation.脂联素、肝激素和肌激素：聚焦它们在脂肪组织炎症中的作用和分子机制。
Front Endocrinol (Lausanne). 2022 Jul 14;13:873699. doi: 10.3389/fendo.2022.873699. eCollection 2022.
9
Review: Vaspin (SERPINA12) Expression and Function in Endocrine Cells.综述：内分秘细胞中 Vaspin（SERPINA12）的表达和功能。
Cells. 2021 Jul 6;10(7):1710. doi: 10.3390/cells10071710.
10
Does a high-fat diet-induced obesity model brown adipose tissue thermogenesis? A systematic review.高脂饮食诱导的肥胖模型会引发棕色脂肪组织产热吗？一项系统评价。
Arch Med Sci. 2019 Jul 22;17(3):596-602. doi: 10.5114/aoms.2019.86781. eCollection 2021.
β-折叠A的碱性残基有助于肝素结合和内脏脂肪素（丝氨酸蛋白酶抑制剂A12）的激活。
J Biol Chem. 2017 Jan 20;292(3):994-1004. doi: 10.1074/jbc.M116.748020. Epub 2016 Dec 9.
4
High fat diet and exercise lead to a disrupted and pathogenic DNA methylome in mouse liver.高脂饮食和运动导致小鼠肝脏中DNA甲基化组紊乱且具有致病性。
Epigenetics. 2017 Jan 2;12(1):55-69. doi: 10.1080/15592294.2016.1261239. Epub 2016 Nov 18.
5
DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes.DNA甲基化抑制3T3-L1脂肪细胞中的瘦素基因。
PLoS One. 2016 Aug 5;11(8):e0160532. doi: 10.1371/journal.pone.0160532. eCollection 2016.
6
Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Three Types of Rat Adipose Tissue.用于三种大鼠脂肪组织定量实时PCR标准化的合适内参基因的筛选
Int J Mol Sci. 2016 Jun 22;17(6):968. doi: 10.3390/ijms17060968.
7
A BAT-Centric Approach to the Treatment of Diabetes: Turn on the Brain.以 BAT 为中心的糖尿病治疗方法：激活大脑。
Cell Metab. 2016 Jul 12;24(1):31-40. doi: 10.1016/j.cmet.2016.05.003. Epub 2016 Jun 9.
8
Epigenetic regulation of KLK7 gene expression in pancreatic and cervical cancer cells.KLK7基因在胰腺癌细胞和宫颈癌细胞中的表观遗传调控
Biol Chem. 2016 Nov 1;397(11):1135-1146. doi: 10.1515/hsz-2015-0307.
9
Transcriptional and epigenetic control of brown and beige adipose cell fate and function.棕色和米色脂肪细胞命运与功能的转录和表观遗传调控。
Nat Rev Mol Cell Biol. 2016 Aug;17(8):480-95. doi: 10.1038/nrm.2016.62. Epub 2016 Jun 2.
10
Central administration of vaspin inhibits glucose production and augments hepatic insulin signaling in high-fat-diet-fed rat.向高脂饮食喂养的大鼠中枢给予内脂素可抑制葡萄糖生成并增强肝脏胰岛素信号传导。
Int J Obes (Lond). 2016 Jun;40(6):947-54. doi: 10.1038/ijo.2016.24. Epub 2016 Feb 12.