Krepela E, Kasafírek E, Novák K, Viklický J
Research Institute of Tuberculosis and Respiratory Diseases, Prague, Czechoslovakia.
Neoplasma. 1990;37(1):61-70.
The occurrence and levels of cathepsin B activity were investigated in primary human lung tumors and lung metastases of renal, colorectal and urinary bladder carcinomas as well as in the associated apparently normal lung parenchyma using a continuous rate enzyme assay with Ac-Leu-Arg-Arg-NHMec (7-(N-acetyl-L-leucyl-L-arginyl-L-arginylamido)-4-methylcoumarin) as the fluorogenic substrate. The inhibition studies of the enzymic hydrolysis of the substrate provided evidence for the catalytic action of the cysteine proteinase cathepsin B (CB) in the lung tumor tissues and the lung parenchyma under the assay conditions used. In the studied group of twenty-four patients with primary lung tumors of all major histological types, the level of CB activity in the tumor tissue was increased twofold and more over that in the associated lung parenchyma in 83% and 75% of cases, when expressed on the basis of wet tissue weight and tissue DNA, respectively. In patients with primary lung adenocarcinoma, the activity of the enzyme in the tumor tissue was elevated over that in the lung parenchyma in all cases studied. In both subgroups of patients with squamous cell lung carcinoma and adenocarcinoma, the mean cathepsin B activity was significantly higher in the tumor tissue than in the lung parenchyma. No obvious correlation was found between the tissue level of cathepsin B activity and the stage of primary lung tumor disease. In a limited number of patients with lung metastases, the level of cathepsin B activity was also higher in the tumor tissue than in the lung parenchyma.
采用以Ac-Leu-Arg-Arg-NHMec（7-(N-乙酰-L-亮氨酰-L-精氨酰-L-精氨酰胺基)-4-甲基香豆素）作为荧光底物的连续速率酶分析法，对原发性人肺肿瘤、肾、结肠和膀胱癌肺转移灶以及相关的貌似正常的肺实质中组织蛋白酶B活性的发生情况和水平进行了研究。底物酶促水解的抑制研究为在所用测定条件下，肺肿瘤组织和肺实质中半胱氨酸蛋白酶组织蛋白酶B（CB）的催化作用提供了证据。在所研究的24例所有主要组织学类型的原发性肺肿瘤患者组中，以湿组织重量和组织DNA为基础分别表示时，肿瘤组织中CB活性水平在83%和75%的病例中比相关肺实质中的活性水平增加了两倍及以上。在原发性肺腺癌患者中，在所研究的所有病例中，肿瘤组织中该酶的活性均高于肺实质中的活性。在肺鳞状细胞癌和腺癌患者的两个亚组中，肿瘤组织中的组织蛋白酶B平均活性均显著高于肺实质中的活性。未发现组织蛋白酶B活性的组织水平与原发性肺肿瘤疾病分期之间存在明显相关性。在有限数量的肺转移患者中，肿瘤组织中的组织蛋白酶B活性水平也高于肺实质中的活性水平。
