Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, University of Concepción, Chile.
Comp Biochem Physiol Part D Genomics Proteomics. 2013 Mar;8(1):17-23. doi: 10.1016/j.cbd.2012.10.004. Epub 2012 Oct 27.
Despite the great relevance of mitochondrial genome analysis in evolutionary studies, there is scarce information on how the transcripts associated with the mitogenome are expressed and their role in the genetic structuring of populations. This work reports the complete mitochondrial genome of the marine gastropod Concholepas concholepas, obtained by 454 pryosequencing, and an analysis of mitochondrial transcripts of two populations 1000 km apart along the Chilean coast. The mitochondrion of C. concholepas is 15,495 base pairs (bp) in size and contains the 37 subunits characteristic of metazoans, as well as a non-coding region of 330 bp. In silico analysis of mitochondrial gene variability showed significant differences among populations. In terms of levels of relative abundance of transcripts associated with mitochondrion in the two populations (assessed by qPCR), the genes associated with complexes III and IV of the mitochondrial genome had the highest levels of expression in the northern population while transcripts associated with the ATP synthase complex had the highest levels of expression in the southern population. Moreover, fifteen polymorphic SNPs were identified in silico between the mitogenomes of the two populations. Four of these markers implied different amino acid substitutions (non-synonymous SNPs). This work contributes novel information regarding the mitochondrial genome structure and mRNA expression levels of C. concholepas.
尽管线粒体基因组分析在进化研究中具有重要意义，但关于与线粒体基因组相关的转录本如何表达及其在种群遗传结构中的作用的信息却很少。本研究报告了通过 454 焦磷酸测序获得的海洋腹足纲动物 Concholepas concholepas 的完整线粒体基因组，并对智利沿海相隔 1000 公里的两个群体的线粒体转录本进行了分析。C. concholepas 的线粒体大小为 15495 个碱基对（bp），包含 37 个后生动物特征的亚基，以及一个 330bp 的非编码区。线粒体基因变异性的计算机分析显示了种群之间的显著差异。就两个群体中与线粒体相关的转录本的相对丰度水平（通过 qPCR 评估）而言，与线粒体基因组复合物 III 和 IV 相关的基因在北部群体中的表达水平最高，而与 ATP 合酶复合物相关的转录本在南部群体中的表达水平最高。此外，还在两个群体的线粒体基因组之间通过计算机鉴定了 15 个多态性单核苷酸多态性（SNP）。其中 4 个标记暗示了不同的氨基酸替换（非同义 SNP）。本研究为 C. concholepas 的线粒体基因组结构和 mRNA 表达水平提供了新的信息。
