蟾毒灵增强吉西他滨对胰腺癌的抗肿瘤作用。
Bufalin enhances the antitumor effect of gemcitabine in pancreatic cancer.
作者信息
Chen Ying, Guo Qingqu, Zhang Bo, Kang Muxing, Xie Qiuping, Wu Yulian
机构信息
Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
出版信息
Oncol Lett. 2012 Oct;4(4):792-798. doi: 10.3892/ol.2012.783. Epub 2012 Jul 2.
Abstract
Bufalin, an active component of the Chinese medicine chan'su, has been reported to have an inhibitory effect on the growth of various types of cancer cells. In the present study, we investigated whether gemcitabine combined with bufalin enhanced the antitumor efficacy in pancreatic cancer. Three pancreatic cancer cell lines (Bxpc-3, Mia PaCa-2 and Panc-1) were treated with gemcitabine and/or bufalin in vitro. The combination treatment demonstrated greater inhibition of cellular growth and apoptosis. The activity of apoptosis signal-regulating kinase 1 (ASK1)/JNK was upregulated in gemcitabine-induced apoptosis when combined with bufalin. We also observed that tumor growth was significantly inhibited by the combination therapy in a tumor-bearing mouse model, and upregulation of ASK1 activity was validated by immunohistochemical staining. These results suggest that bufalin may be a potential chemotherapeutic agent for pancreatic cancer, which could enhance the antitumor efficacy of gemcitabine when used in combination, possibly through the activation of ASK1/JNK.
摘要
蟾酥灵是中药蟾酥的一种活性成分，据报道对多种癌细胞的生长具有抑制作用。在本研究中，我们调查了吉西他滨联合蟾酥灵是否能增强对胰腺癌的抗肿瘤疗效。三种胰腺癌细胞系（Bxpc-3、Mia PaCa-2和Panc-1）在体外接受了吉西他滨和/或蟾酥灵的处理。联合治疗显示出对细胞生长和凋亡的更强抑制作用。与蟾酥灵联合使用时，凋亡信号调节激酶1（ASK1）/JNK的活性在吉西他滨诱导的凋亡中上调。我们还观察到，在荷瘤小鼠模型中，联合治疗显著抑制了肿瘤生长，并且通过免疫组化染色证实了ASK1活性的上调。这些结果表明，蟾酥灵可能是一种潜在的胰腺癌化疗药物，与吉西他滨联合使用时可增强抗肿瘤疗效，可能是通过激活ASK1/JNK来实现的。
相似文献
1
Bufalin enhances the antitumor effect of gemcitabine in pancreatic cancer.蟾毒灵增强吉西他滨对胰腺癌的抗肿瘤作用。
Oncol Lett. 2012 Oct;4(4):792-798. doi: 10.3892/ol.2012.783. Epub 2012 Jul 2.
2
Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1α/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells.沉默c-Myc通过抑制胰腺癌细胞中的HIF-1α/SDF-1/CXCR4通路增强蟾蜍灵的抗肿瘤活性。
Front Pharmacol. 2020 Apr 17;11:495. doi: 10.3389/fphar.2020.00495. eCollection 2020.
3
Synergistic antitumor activity of gemcitabine combined with triptolide in pancreatic cancer cells.吉西他滨联合雷公藤内酯醇对胰腺癌细胞的协同抗肿瘤活性
Oncol Lett. 2016 May;11(5):3527-3533. doi: 10.3892/ol.2016.4379. Epub 2016 Mar 29.
4
Selective inhibitory effect of bufalin on growth of human tumor cells in vitro: association with the induction of apoptosis in leukemia HL-60 cells.蟾毒灵对人肿瘤细胞体外生长的选择性抑制作用：与白血病HL-60细胞凋亡诱导的相关性。
Jpn J Cancer Res. 1994 Jun;85(6):645-51. doi: 10.1111/j.1349-7006.1994.tb02408.x.
5
Involvement of Tiam1 in apoptosis induced by bufalin in HeLa cells.Tiam1在蟾蜍灵诱导HeLa细胞凋亡中的作用
Anticancer Res. 2007 Jan-Feb;27(1A):245-9.
6
High-intensity focused ultrasound enhances the effect of bufalin by inducing apoptosis in pancreatic cancer cells.高强度聚焦超声通过诱导胰腺癌细胞凋亡增强了蟾毒灵的作用。
Onco Targets Ther. 2019 Feb 12;12:1161-1170. doi: 10.2147/OTT.S185953. eCollection 2019.
7
Tiam1 is involved in the regulation of bufalin-induced apoptosis in human leukemia cells.Tiam1参与调控蟾蜍灵诱导的人白血病细胞凋亡。
Oncogene. 1999 Apr 15;18(15):2413-21. doi: 10.1038/sj.onc.1202555.
8
Gemcitabine‑fucoxanthin combination in human pancreatic cancer cells.吉西他滨与岩藻黄质联合作用于人类胰腺癌细胞
Biomed Rep. 2023 Jun 1;19(1):46. doi: 10.3892/br.2023.1629. eCollection 2023 Jul.
9
Down-regulation of Cbl-b by bufalin results in up-regulation of DR4/DR5 and sensitization of TRAIL-induced apoptosis in breast cancer cells.蟾毒灵下调 Cbl-b 导致乳腺癌细胞中 DR4/DR5 的上调和 TRAIL 诱导的细胞凋亡敏感性增加。
J Cancer Res Clin Oncol. 2012 Aug;138(8):1279-89. doi: 10.1007/s00432-012-1204-4. Epub 2012 Mar 24.
10
Synergistic effect between erlotinib and MEK inhibitors in KRAS wild-type human pancreatic cancer cells.厄洛替尼与 MEK 抑制剂在 KRAS 野生型人胰腺癌细胞中的协同作用。
Clin Cancer Res. 2011 May 1;17(9):2744-56. doi: 10.1158/1078-0432.CCR-10-2214. Epub 2011 Mar 8.
引用本文的文献
1
Combinational Antitumor Strategies Based on the Active Ingredients of Toad Skin and Toad Venom.基于蟾皮和蟾毒液活性成分的联合抗肿瘤策略。
Drug Des Devel Ther. 2024 Aug 9;18:3549-3594. doi: 10.2147/DDDT.S469832. eCollection 2024.
2
Global trends in bufalin application research for cancer from 2003 to 2022: A bibliometric and visualised analysis.2003年至2022年蟾毒灵在癌症应用研究中的全球趋势：文献计量与可视化分析
Heliyon. 2024 Jan 9;10(2):e24395. doi: 10.1016/j.heliyon.2024.e24395. eCollection 2024 Jan 30.
3
An overview of the past decade of bufalin in the treatment of refractory and drug-resistant cancers: current status, challenges, and future perspectives.蟾毒灵治疗难治性和耐药性癌症的十年综述：现状、挑战及未来展望
Front Pharmacol. 2023 Oct 4;14:1274336. doi: 10.3389/fphar.2023.1274336. eCollection 2023.
4
Advances in the treatment of pancreatic cancer with traditional Chinese medicine.胰腺癌的中医治疗进展
Front Pharmacol. 2023 Aug 7;14:1089245. doi: 10.3389/fphar.2023.1089245. eCollection 2023.
5
Emergence of Cardiac Glycosides as Potential Drugs: Current and Future Scope for Cancer Therapeutics.心脏糖苷作为潜在药物的出现：癌症治疗的当前和未来范围。
Biomolecules. 2021 Aug 25;11(9):1275. doi: 10.3390/biom11091275.
6
Effects of low-dose bufalin combined with hydroxycamptothecin on human castration-resistant prostate cancer xenografts in nude mice.低剂量蟾毒灵联合羟基喜树碱对裸鼠人去势抵抗性前列腺癌异种移植瘤的影响
Exp Ther Med. 2021 Sep;22(3):1015. doi: 10.3892/etm.2021.10447. Epub 2021 Jul 15.
7
New therapeutic aspects of steroidal cardiac glycosides: the anticancer properties of Huachansu and its main active constituent Bufalin.甾体强心苷的新治疗领域：华蟾素及其主要活性成分蟾毒灵的抗癌特性。
Cancer Cell Int. 2019 Apr 11;19:92. doi: 10.1186/s12935-019-0806-1. eCollection 2019.
8
Bufalin enhances antitumor effect of paclitaxel on cervical tumorigenesis via inhibiting the integrin α2/β5/FAK signaling pathway.蟾毒灵通过抑制整合素α2/β5/黏着斑激酶信号通路增强紫杉醇对宫颈癌发生的抗肿瘤作用。
Oncotarget. 2016 Feb 23;7(8):8896-907. doi: 10.18632/oncotarget.6840.
9
Evaluating the cancer therapeutic potential of cardiac glycosides.评估强心苷的癌症治疗潜力。
Biomed Res Int. 2014;2014:794930. doi: 10.1155/2014/794930. Epub 2014 May 8.
10
Bufalin inhibits the differentiation and proliferation of human osteosarcoma cell line hMG63-derived cancer stem cells.蟾毒灵抑制人骨肉瘤细胞系hMG63来源的癌症干细胞的分化和增殖。
Tumour Biol. 2014 Feb;35(2):1075-82. doi: 10.1007/s13277-013-1143-y. Epub 2013 Sep 5.
本文引用的文献
1
Bufalin induces autophagy-mediated cell death in human colon cancer cells through reactive oxygen species generation and JNK activation.蟾毒灵通过产生活性氧自由基和激活 JNK 诱导人结肠癌细胞自噬性死亡。
Free Radic Biol Med. 2011 Oct 1;51(7):1365-75. doi: 10.1016/j.freeradbiomed.2011.06.016. Epub 2011 Jun 29.
2
Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells.蟾毒灵诱导 ASTC-a-1 细胞中活性氧依赖的 Bax 易位和细胞凋亡。
Evid Based Complement Alternat Med. 2011;2011:249090. doi: 10.1093/ecam/nep082. Epub 2011 Jun 18.
3
Depleting MEKK1 expression inhibits the ability of invasion and migration of human pancreatic cancer cells.耗尽MEKK1表达会抑制人胰腺癌细胞的侵袭和迁移能力。
J Cancer Res Clin Oncol. 2009 Dec;135(12):1655-63. doi: 10.1007/s00432-009-0612-6. Epub 2009 Jun 10.
4
Thrombin-induced connective tissue growth factor expression in human lung fibroblasts requires the ASK1/JNK/AP-1 pathway.凝血酶诱导人肺成纤维细胞中结缔组织生长因子的表达需要ASK1/JNK/AP-1信号通路。
J Immunol. 2009 Jun 15;182(12):7916-27. doi: 10.4049/jimmunol.0801582.
5
Increased nuclear expression and transactivation of vitamin D receptor by the cardiotonic steroid bufalin in human myeloid leukemia cells.强心甾类化合物蟾毒灵在人髓性白血病细胞中增强维生素D受体的核表达及反式激活作用
J Steroid Biochem Mol Biol. 2009 Apr;114(3-5):144-51. doi: 10.1016/j.jsbmb.2009.01.022. Epub 2009 Feb 6.
6
Potentiation of the effect of gemcitabine by emodin in pancreatic cancer is associated with survivin inhibition.大黄素增强吉西他滨对胰腺癌的疗效与抑制生存素有关。
Biochem Pharmacol. 2009 Jun 1;77(11):1674-83. doi: 10.1016/j.bcp.2009.02.021. Epub 2009 Mar 11.
7
Apoptosis signal-regulating kinase 1 mediates denbinobin-induced apoptosis in human lung adenocarcinoma cells.凋亡信号调节激酶1介导地蒽酚诱导的人肺腺癌细胞凋亡。
J Biomed Sci. 2009 May 1;16(1):43. doi: 10.1186/1423-0127-16-43.
8
Apoptotic signaling in bufalin- and cinobufagin-treated androgen-dependent and -independent human prostate cancer cells.蟾毒灵和华蟾酥毒基处理雄激素依赖和非依赖型人前列腺癌细胞中的凋亡信号传导
Cancer Sci. 2008 Dec;99(12):2467-76. doi: 10.1111/j.1349-7006.2008.00966.x. Epub 2008 Nov 26.
9
Bufalin induces growth inhibition, cell cycle arrest and apoptosis in human endometrial and ovarian cancer cells.蟾毒灵可诱导人子宫内膜癌细胞和卵巢癌细胞生长抑制、细胞周期阻滞及凋亡。
Int J Mol Med. 2008 May;21(5):637-43.
10
Enhanced anti-tumor effect of combination therapy with gemcitabine and apigenin in pancreatic cancer.吉西他滨与芹菜素联合治疗对胰腺癌的抗肿瘤作用增强。
Cancer Lett. 2008 Jan 18;259(1):39-49. doi: 10.1016/j.canlet.2007.09.015. Epub 2007 Oct 29.
Zotero 插件