Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, Center for Molecular Biology, University of Vienna, A-1030 Vienna, Austria.
J Integr Plant Biol. 2013 Jan;55(1):75-82. doi: 10.1111/jipb.12016.
Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.
小泛素样修饰物（SUMO）缀合影响植物的广泛过程，包括生长、花起始、病原体防御和对非生物胁迫的响应。在这里，我们研究了一种在活性位点发生 Cys 到 Ser 变化的 SUMO 连接酶的体内和体外情况，并表明它具有显性负效应。在植物体内表达会显著扰乱正常发育，导致生长迟缓、早花和基因表达变化。我们认为，突变蛋白可以作为研究 SUMO 化的探针，对于遗传基础设施较差、无法通过功能缺失突变体进行分析的植物也是如此。
