Wu Xu, Xu Xiao, Lu Qun, Dai Yu-ping, Wu Zhong-ming
Department of Gastroenterology, Zunyi Medical College, Zunyi, China.
Zhonghua Gan Zang Bing Za Zhi. 2012 Nov;20(11):817-21. doi: 10.3760/cma.j.issn.1007-3418.2012.11.004.
To examine the differential levels of fecal Bifidobacterium, Bacteroides, Eubacterium rectale-Clostridium, Escherichia coli, Enterococcus, and Clostridium difficile between patients with hepatic cirrhosis and healthy controls. Fecal samples were collected from 29 patients with hepatic cirrhosis treated in the Department of Digestive Diseases at Zunyi Hospital between March and December of 2010.
Fecal samples were collected from 13 healthy college students for use as controls. All samples were assessed by pH measurement, bacterial culture for turbidity, fluorescence in situ hybridization, and laser scanning confocal microscopy. The t-test and rank correlation test were used to determine statistical significance of intergroup differences in each tested parameter.
The feces of patients with hepatic cirrhosis had higher pH than that of healthy controls (6.79+/-0.64 vs. 6.18+/-0.74, P less than 0.05). The bacterial turbidity was not significantly different between the feces of hepatic cirrhosis patients and healthy controls (1.15+/-0.59 vs. 1.39+/-1.01, P more than 0.05). The numbers of Bifidobacterium, Bacteroides, Eubacterium rectale-Clostridium, Escherichia coli, Enterococcus, and Clostridium difficile in feces of patients with hepatic cirrhosis were significantly lower than those of the controls (all P less than 0.01). No significant correlation was found between the number or ratio of bacteria species and the severity of hepatic cirrhosis (Child-Pugh scores; P more than 0.05).
The total quantity of intestinal bacteria in patients with hepatic cirrhosis is not significantly different from that in healthy patients. However, the profile of intestinal bacteria is different, which may explain the increased pH of fecal samples from patients with hepatic cirrhosis, but the differential profile is not correlated to cirrhosis pathogenesis.
检测肝硬化患者与健康对照者粪便中双歧杆菌、拟杆菌、直肠真杆菌-梭菌、大肠杆菌、肠球菌及艰难梭菌的差异水平。于2010年3月至12月期间，从遵义医院消化内科收治的29例肝硬化患者中采集粪便样本。
采集13名健康大学生的粪便样本作为对照。所有样本均通过pH值测定、细菌培养浊度分析、荧光原位杂交及激光扫描共聚焦显微镜进行评估。采用t检验和秩相关检验确定各检测参数组间差异的统计学意义。
肝硬化患者粪便pH值高于健康对照者（6.79±0.64 vs. 6.18±0.74，P<0.05）。肝硬化患者粪便与健康对照者粪便的细菌浊度无显著差异（1.15±0.59 vs. 1.39±1.01，P>0.05）。肝硬化患者粪便中双歧杆菌、拟杆菌、直肠真杆菌-梭菌、大肠杆菌、肠球菌及艰难梭菌的数量均显著低于对照组（均P<0.01）。细菌种类数量或比例与肝硬化严重程度（Child-Pugh评分）之间未发现显著相关性（P>0.05）。
肝硬化患者肠道细菌总量与健康患者无显著差异。然而，肠道细菌谱不同，这可能解释了肝硬化患者粪便样本pH值升高的原因，但差异谱与肝硬化发病机制无关。
