Valdenegro-Vega Victoria A, Crosbie Philip, Vincent Benita, Cain Kenneth D, Nowak Barbara F
NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, Australia.
Vet Immunol Immunopathol. 2013 Jan 15;151(1-2):113-23. doi: 10.1016/j.vetimm.2012.10.010. Epub 2012 Nov 7.
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens - dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) - administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.
本研究旨在评估大西洋鲑（Salmo salar）在采用不同递送策略给予两种蛋白-半抗原抗原（二硝基苯酚（DNP）和异硫氰酸荧光素（FITC），均与钥孔戚血蓝蛋白（KLH）偶联）时的全身和黏膜免疫反应。鱼通过不同途径接触抗原，并在初次接触后4周给予加强剂量。使用酶联免疫吸附测定（ELISA）在12周内测量全身和黏膜抗体反应。只有通过腹腔内（IP）注射接触两种抗原的鱼在免疫后6周开始显示全身抗体反应增加。没有任何处理能够产生黏膜抗体反应；然而，从注射FITC的鱼在免疫后12周获得的皮肤外植体的组织上清液中抗体水平有所增加。用皮肤外植体的血清和培养上清液进行的蛋白质印迹显示了针对抗原的特异性反应。总之，在大西洋鲑中腹腔内注射半抗原-抗原是诱导该物种针对这些抗原产生抗体反应的最佳递送途径。尽管腹腔内注射未导致皮肤黏液中抗体水平增加，但全身抗体反应增加，并且如组织外植体上清液中特异性抗体水平升高所示，黏膜组织中的抗体产生明显增加。
