RIKILT, Institute of Food Safety, Wageningen, The Netherlands.
Anal Bioanal Chem. 2013 Feb;405(5):1631-9. doi: 10.1007/s00216-012-6591-5. Epub 2012 Dec 5.
Bacterial products based on Bacillus thuringiensis are registered in many countries as plant protection products (PPPs) and are widely used as insecticides and nematocides. However, certain B. thuringiensis strains produce harmful toxins and are therefore not allowed to be used as PPPs. The serotype B. thuringiensis thuringiensis produces the beta-exotoxin thuringiensin (ßeT) which is considered to be toxic for almost all forms of life including humans (WHO 1999). The use of a non-registered PPP based on B. thuringiensis thuringiensis called bitoxybacillin was established through the determination of ßeT. First, an analytical reference standard of ßeT was characterized by nuclear magnetic resonance, liquid chromatography-high-resolution mass spectrometry and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Then, a confirmatory quantitative method for the determination of ßeT in PPPs and selected greenhouse crops based on LC-MS/MS was developed and validated. A limit of quantitation of 0.028 mg/kg was established, and average recoveries ranged from 85.6 % to 104.8 % with repeatability (RSDr) of 1.5-7.7 % and within-lab reproducibility (RSD(WLR)) of 17 %. The method was used for analysis of >100 samples. ßeT was found in leaves of ornamentals, but no evidence was found for use in edible crops.
基于苏云金芽孢杆菌的细菌产品已在许多国家注册为植物保护产品 (PPPs)，并被广泛用作杀虫剂和杀线虫剂。然而，某些苏云金芽孢杆菌菌株会产生有害毒素，因此不允许将其用作 PPPs。苏云金芽孢杆菌血清型 B. thuringiensis 产生β-外毒素苏云金菌素 (ßeT)，被认为对包括人类在内的几乎所有生命形式都有毒（世界卫生组织，1999 年）。一种名为 bitoxybacillin 的非注册基于苏云金芽孢杆菌的 PPP 的使用是通过确定ßeT 来确定的。首先，通过核磁共振、液相色谱-高分辨率质谱和液相色谱-串联质谱 (LC-MS/MS) 对ßeT 的分析参考标准进行了表征。然后，开发并验证了基于 LC-MS/MS 的 PPP 和选定温室作物中ßeT 的定量确证方法。建立了定量限为 0.028 mg/kg，平均回收率为 85.6%至 104.8%，重复性 (RSDr) 为 1.5-7.7%，实验室内重现性 (RSD(WLR)) 为 17%。该方法用于分析了 >100 个样品。ßeT 在观赏植物的叶片中被发现，但没有证据表明其在食用作物中使用。
