Liu C M, Tzeng Y M
Institute of Biotechnology, National Dong Hwa University, Shoufeng, Taiwan, Republic of China.
J Chromatogr Sci. 1998 Jul;36(7):340-4. doi: 10.1093/chromsci/36.7.340.
An analytical method for thuringiensin using adenosine monophosphate (AMP) as an internal standard is established. AMP, with high stability and availability, is an appropriate internal standard for thuringiensin quantitative analysis using high-performance liquid chromatography and ultraviolet absorbance detection at 260 nm. A good correlation between the concentration of thuringiensin and the peak-area ratios of thuringiensin to AMP is demonstrated. From this general equation of linear regression line, the concentration of thuringiensin can be assessed in fermentation broth or semi-purified product.
建立了一种以腺苷一磷酸（AMP）为内标物测定苏云金菌素的分析方法。AMP稳定性高且易于获得，是采用高效液相色谱法和260nm紫外吸光度检测进行苏云金菌素定量分析的合适内标物。结果表明，苏云金菌素浓度与苏云金菌素和AMP的峰面积比之间具有良好的相关性。根据该线性回归线的一般方程，可以评估发酵液或半纯化产物中苏云金菌素的浓度。
