Department of Biosciences, Federal University of São Paulo, São Paulo SP 11060-001, Brazil.
Toxicol Mech Methods. 2013 May;23(4):289-96. doi: 10.3109/15376516.2012.755594. Epub 2013 Jan 16.
The objective of this article was to evaluate the impact potential of nandrolone decanoate on DNA damage, cellular regulatory proteins and cyclooxygenase (COX)-2 in oral mucosa cells of Wistar rats. A total of 40 rats were distributed into four groups. Two experimental groups were treated with nandrolone decanoate, at 5 mg/kg doses, subcutaneously, three times a week in two periods: 15 and 30 days. The remaining groups received only 0.9% saline subcutaneously, three times a week. To evaluate genetic damage, nandrolone decanoate at 15 mg/kg dose was exposed to 24 h. In the histopathological analysis, no remarkable morphological changes were observed in tongue tissue in all groups. Significant increase in immunoexpression of Ki-67, p53, COX-2 proteins was detected in the groups treated with nandrolone decanoate during 15 and 30 days, when compared to their respective controls. A positive correlation between immunoexpression of p53 and COX-2 protein was detected following nandrolone decanoate exposure. DNA damage was induced by nandrolone decanoate in oral mucosa cells at 15 mg/kg dose. Our results suggest that nandrolone decanoate was able to alter the expression of cell cycle-related proteins, as well as to induce genetic damage and COX-2 immunoexpression in tongue cells of Wistar rats.
本文旨在评估癸酸诺龙对 Wistar 大鼠口腔黏膜细胞 DNA 损伤、细胞调节蛋白和环氧化酶（COX）-2 的潜在影响。将 40 只大鼠随机分为四组。两组实验组以 5mg/kg 的剂量，每周三次，皮下注射癸酸诺龙，分为两个周期：15 天和 30 天。其余两组仅每周三次接受 0.9%生理盐水皮下注射。为了评估遗传损伤，将 15mg/kg 剂量的癸酸诺龙暴露于 24 小时。在组织病理学分析中，所有组的舌组织均未观察到明显的形态变化。与各自的对照组相比，癸酸诺龙治疗 15 天和 30 天的组中，Ki-67、p53、COX-2 蛋白的免疫表达显著增加。癸酸诺龙暴露后，p53 和 COX-2 蛋白的免疫表达呈正相关。癸酸诺龙在 15mg/kg 剂量下诱导了口腔黏膜细胞的 DNA 损伤。我们的结果表明，癸酸诺龙能够改变与细胞周期相关的蛋白表达，并诱导 Wistar 大鼠舌细胞的遗传损伤和 COX-2 免疫表达。
