1,5-碘代萘基叠氮化物对基孔肯雅病毒的灭活作用。
Inactivation of Chikungunya virus by 1,5 iodonapthyl azide.
机构信息
Department of Pathology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
出版信息
Virol J. 2012 Dec 4;9:301. doi: 10.1186/1743-422X-9-301.
BACKGROUND
Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia.
FINDINGS
In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis.
CONCLUSION
INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine.
背景
基孔肯雅病毒（CHIKV）是一种虫媒传播的甲病毒，属于披膜病毒科。CHIKV 是一种新出现的病毒，目前尚无安全的预防性疫苗。此前，CHIKV 的一种减毒活株 CHIK181/25 在人类中表现出高度的免疫原性，但仍有残余的毒力，导致短暂的关节痛。
结果
在这项研究中，我们证明了 1,5 碘代萘基叠氮化物（INA）可完全使 CHIKV181/25 失活。感染 INA 灭活 CHIKV 的细胞未观察到细胞病变效应和病毒复制。然而，通过 Western blot 分析观察到 INA 灭活的 CHIK 病毒-抗体结合能力降低。
结论
INA 完全使 CHIKV 失活，可进一步探索用于开发灭活 CHIKV 疫苗。
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