放射性免疫治疗利用 BR96 抗体后同种大鼠结肠癌转移灶中抗原表达的模式。

Pattern of antigen expression in metastases after radioimmunotherapy of a syngeneic rat colon carcinoma utilizing the BR96 antibody.

机构信息

Department of Oncology, Lund University, Lund, Sweden.

Department of Medical Radiation Physics, Lund University, Lund, Sweden.

出版信息

Exp Hematol Oncol. 2012 Nov 13;1(1):34. doi: 10.1186/2162-3619-1-34.

DOI:10.1186/2162-3619-1-34

PMID:23210841

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3514130/

Abstract

BACKGROUND

Repeated administration of antibody-based therapies such as radioimmunotherapy depends on preserved antigen expression in tumor lesions. The purpose of this study was to evaluate whether the antigen expression in metastases observed after radioimmunotherapy differs from that of untreated primary tumors.

FINDINGS

30 of the 35 Brown Norway rats with syngeneic colon carcinoma treated with 400 MBq/kg 177Lu-DOTA-BR96 exhibited consistent complete response of the primary tumor. 13 animals developed metastases that were detected after treatment. The antigen expression was reduced in 17 of 23 metastases detected after radioimmunotherapy compared with untreated tumors. No tumors completely lacked positively stained tumor cells.

CONCLUSIONS

Although it was not possible to demonstrate that the antigen reduction is triggered by the radioimmunotherapy this result stress the importance of considering the risk of reduced antigen expression in metastases after radioimmunotherapy prior to further targeted therapies.

摘要

背景

基于抗体的治疗方法（如放射免疫疗法）的重复给药取决于肿瘤病变中抗原表达的保留。本研究的目的是评估放射免疫治疗后观察到的转移灶中的抗原表达是否与未经治疗的原发性肿瘤不同。

结果

35 只接受 400MBq/kg177Lu-DOTA-BR96 治疗的同基因结肠癌细胞的褐鼠中，有 30 只原发性肿瘤表现出一致的完全缓解。13 只动物发生了转移，在治疗后被检测到。与未经治疗的肿瘤相比，在放射免疫治疗后检测到的 23 个转移灶中有 17 个的抗原表达减少。没有肿瘤完全缺乏阳性染色的肿瘤细胞。