Key Laboratory of Molecular Biology, Hainan Medical College, Haikou.
Cancer Lett. 2013 Apr 28;330(2):170-80. doi: 10.1016/j.canlet.2012.11.042. Epub 2012 Dec 2.
AFP and its receptor (AFPR) are early indicators of HBx-driven hepatocarcinogenesis. Clinical specimens, normal human liver cells L-02 and HCC cell lines were selected for analyzing the effects of HBx on expression of AFP, AFPR, Src, CXCR4, and Ras. Results showed that AFPR localized in the membranes of HCC samples. HBx upregulated the expression of AFPR and AFP prior to expression of Src, CXCR4, and Ras in L-02 cells and in liver specimens; Target-labeled AFPR was able to reveal the location and metastatic status of HCC in vivo. In this way, actuated expression of AFPR served as an indicator suitable for use in the early diagnosis of HBx-driven malignant transformation of hepatocytes. Labeled AFPR may be applied to trace primary and metastatic HCC.
甲胎蛋白及其受体（AFPR）是 HBx 驱动的肝癌发生的早期指标。选择临床标本、正常人肝细胞 L-02 和肝癌细胞系来分析 HBx 对 AFP、AFPR、Src、CXCR4 和 Ras 表达的影响。结果表明，AFPR 定位于 HCC 样本的膜上。在 L-02 细胞和肝标本中，HBx 上调了 AFP 和 AFPR 的表达，然后上调了 Src、CXCR4 和 Ras 的表达；靶向标记的 AFPR 能够揭示 HCC 在体内的位置和转移状态。这样，AFPR 的激活表达可作为用于早期诊断 HBx 驱动的肝细胞恶性转化的指标。标记的 AFP 可能适用于追踪原发性和转移性 HCC。
