Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra, Portugal.
Invest Ophthalmol Vis Sci. 2013 Jan 17;54(1):429-43. doi: 10.1167/iovs.12-10776.
Neuropeptide Y (NPY) is one of the most abundant peptides in the central nervous system (CNS), including the retina. This peptide activates various different G-coupled receptors (NPY Y(1), Y(2), Y(4), and Y(5)) that are also present in the retina. However, the localization of NPY receptors in the several types of retinal cells is not completely known. In this study, we have looked at the distribution of NPY Y(1) and Y(2) receptors in rat retinal cells to reveal new perspectives on the role of NPY receptors in retina physiology.
Rat retinal neural cell cultures were prepared from newborn Wistar rats (P3-P5) and pure rat Müller cell culture was obtained after treatment of these cells with ascorbic acid. The presence of NPY Y(1) and Y(2) in retinal cell types was studied by immunocytochemistry.
We show that NPY Y(1) and Y(2) receptors are present on every cell type of rat retinal cell cultures. Neurons, as photoreceptors, bipolar, horizontal, amacrine, and ganglion cells, express these two types of NPY receptors. NPY Y(1) and Y(2) receptors are also located in macroglial cells (Müller cells and astrocytes) and microglial cells.
We have clarified the presence of the NPY Y(1) and Y(2) receptors in all different cell types that constitute the retina, which we believe will help open new perspectives for studying the physiology and the potential pathophysiologic function of NPY and its receptors in the retina.
神经肽 Y（NPY）是中枢神经系统（CNS）中最丰富的肽之一，包括视网膜。这种肽激活了各种不同的 G 蛋白偶联受体（NPY Y(1)、Y(2)、Y(4)和 Y(5)），这些受体也存在于视网膜中。然而，NPY 受体在几种视网膜细胞中的定位尚不完全清楚。在这项研究中，我们研究了 NPY Y(1)和 Y(2)受体在大鼠视网膜细胞中的分布，以揭示 NPY 受体在视网膜生理学中的新作用。
从新生 Wistar 大鼠（P3-P5）中制备大鼠视网膜神经细胞培养物，并在用抗坏血酸处理这些细胞后获得纯大鼠 Müller 细胞培养物。通过免疫细胞化学研究 NPY Y(1)和 Y(2)受体在视网膜细胞类型中的存在。
我们表明，NPY Y(1)和 Y(2)受体存在于大鼠视网膜细胞培养物的每种细胞类型上。神经元，作为光感受器、双极、水平、无长突和节细胞，表达这两种类型的 NPY 受体。NPY Y(1)和 Y(2)受体也位于大胶质细胞（Müller 细胞和星形胶质细胞）和小胶质细胞中。
我们已经阐明了 NPY Y(1)和 Y(2)受体存在于构成视网膜的所有不同细胞类型中，我们相信这将有助于为研究 NPY 及其受体在视网膜中的生理学和潜在病理生理功能开辟新的视角。
