MIGAL-Galilee Technology Center, Kiryat Shmona 11016, Israel.
Vaccine. 2013 Jan 11;31(4):626-31. doi: 10.1016/j.vaccine.2012.11.058. Epub 2012 Dec 2.
Passive vaccination is used to treat a wide range of infections and cancer. However, this approach has some limitations. An immune complex termed Y-complex was developed to intensify the effect of the passive vaccine. The complex is composed of a microbead that carries specific antibodies and an inducer. It enables targeting of pathogen or abnormal cells, and stimulation of a desired response by innate immune cells, depending on the inducer. The production and efficacy of Y-complex as a passive immune prophylaxis is demonstrated in this study by its use in treating cow mastitis. In an in vitro assay, Y-complex inhibited propagation and induced phagocytosis of bacteria. In challenge experiments, cows were inoculated through the udder with Escherichia coli or Streptococcus dysgalactiae. Following treatment with Y-complex, no bacteria were isolated in the milk and N-acetyl-ß-D-glucosaminidase activity had returned to normal levels. Thus the Y-complex approach can be used as an effective treatment for mastitis. Due to its modularity, this approach may serve as a treatment for a variety of disease agents.
被动免疫接种被用于治疗多种感染和癌症。然而，这种方法存在一些局限性。一种名为 Y 复合物的免疫复合物被开发出来以增强被动疫苗的效果。该复合物由携带特定抗体和诱导剂的微珠组成。它能够根据诱导剂靶向病原体或异常细胞，并刺激先天免疫细胞产生所需的反应。本研究通过在治疗奶牛乳腺炎中的应用，证明了 Y 复合物作为被动免疫预防的生产和功效。在体外试验中，Y 复合物抑制了细菌的繁殖并诱导了细菌的吞噬作用。在攻毒实验中，奶牛通过乳房接种大肠杆菌或无乳链球菌。用 Y 复合物处理后，牛奶中未分离出细菌，N-乙酰-β-D-氨基葡萄糖苷酶活性已恢复正常水平。因此，Y 复合物方法可用于乳腺炎的有效治疗。由于其模块化，这种方法可以作为多种疾病病原体的治疗方法。
